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Recombinant Human CD177 Protein, His tag, 10x 100µg  

Recombinant Human CD177 Protein, His tag, 10x 100µg

Recombinant Human CD177 Protein, AA Leu 22 - Gly 407, expressed from human 293 cells (HEK293), His Tag

Synonym
Recombinant human protein, CD177, PRV1, NB1, HNA-2a, NB1 GP

More details

CD7-H5223-1K

Availability: within 7 days

1 600,00 €

Background
CD antigen CD177 is also known as Polycythemia rubra vera protein 1 (PRV-1), Human neutrophil alloantigen 2a, NB1 glycoprotein, is a glycosyl-phosphatidylinositol (GPI)-linked N-glycosylated cell surface glycoprotein. CD177 contains two UPAR/Ly6 domains. CD177 is highly expressed in normal bone marrow and weakly expressed in fetal liver. PECAM-1 is a binding partner of CD177.

Source
Human CD177, His Tag (CD7-H5223) is expressed from human 293 cells (HEK293). It contains AA Leu 22 - Gly 407 (Accession # Q8N6Q3-1).
Predicted N-terminus: Leu 22

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 43.2 kDa. The protein migrates as 50-57 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

References

(1) Temerinac S., et al., 2000, Blood 95:2569-2576.
(2) Zhang Z., et al., 2004, Protein Sci. 13:2819-2824.
(3) Klippel S., et al., 2002, Blood 100:2441-2448.