Recombinant human CDK1/CycA, active protein kinase,10 µg

Cdks are the catalytic subunits of serine/threonine protein kinases which are involved in the progression through the cell cycle. The catalytic activity of these kinases requires interaction with cyclins, which are synthesized and degraded during the cell cycle in a cyclical fashion. At the end of the S-phase A type cyclins associated with CDK1 and CDK2 phosphorylate substrates which are involved in DNA replication (MCMs and Cdc7). During G2 phase Cyclin A is degraded whereas Cyclin B is actively synthesized. As a consequence, CDK1 binds to Cyclin B, an important step in triggering mitosis.

Recombinant human cyclin-dependent protein kinase CDK1 (cdc2), coexpressed in complex with Cyclin A2.

Theoretical MW GST-CDK1: 63.9 kDa
Theoretical MW GST-CycA2: 75.0 kDa
Expression system: Baculovirus infected Sf9 cells
Storage buffer: 50 mM HEPES pH 7.5,100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20%
 glycerol
Protein concentration: 0.126 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MSFC membrane
Specific activity: 8,000 pmol/mg x min

Ordering information: shipped on dry ice

Entrez Gene ID: 983/890 
UniProtKB:  P06493/P20248

Product specific literature references:

Sanchez I, Dynlacht BD. (2005) "New insights into cyclins, CDKs, and cell cycle control."
Semin Cell Dev Biol. 16 (3):311-21. 

Malumbres M, Barbacid M. (2005) �Mammalian cyclin-dependent kinases."Trends Biochem Sci.,30(11):630-41. 

Koseoglu MM, Graves LM, Marzluff WF. (2008) � Phosphorylation of threonine 61 by cyclin a/Cdk1 triggers degradation of stem-loop binding protein at the end of S phase." Mol Cell Biol. 28(14):4469-79.