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The Cdks are serine/threonine protein kinases which are predominantly involved in the progression of the cell cycle. Subsequently, CDK-cyclin complexes (CDK7, CDK8 and CDK9) were also identified as conserved components of the RNA polymerase II transcriptional machinery. CDK7, cyclin H and a third subunit, the RING finger protein MAT1 associated with transcription factor TFIIH and phosphorylates the C-terminal domain (CTD) of RNA polymerase II, resulting in a progression of transcription from the preinitiation to the initiation stage. Additionally, CDK7 together with CycH and MAT1 forms the CDK activating kinase (CAK) complex, responsible for the activating phosphorylation within the T-loop of CDK1, CDK2, CDK4 and CDK6.
Reombinant human cyclin-dependent protein kinase CDK7, coexpressed in complex with with human CycH and human MAT1. Protein: Triple expression of human CDK7, amino acids M1-F346N-terminally fused to GST-HIS6-Thrombin cleavage site and human CycH,amino acids M1-L323 and human MAT1, amino acids M1-S309 both N-terminally fused to HIS 6-Thrombin cleavage site
Theoretical MW MWGST-CDK7: 68.9 kDa
Theoretical MWCycH: 42.4 kDa
Theoretical MWMAT1: 40.6 kDa
Expression system: Baculovirus infected Sf9 cells
Purification: One-step affinity purification using glutathione agarose
Storage buffer: 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol
Protein concentration: 0.139 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MAFC membrane
Specific activity: 22,000 pmol/mg x min
Entrez Gene ID: 1022/902/4331
UniProtKB: P50613/P51946/P51948
Ordering information: shipped on dry ice
Fisher RP. (2005) "Secrets of a double agent: CDK7 in cell-cycle control and transcription." J Cell Sci. 15; 118(Pt 22):5171-80.
Lolli G, Johnson LN. (2005) "CAK-Cyclin-dependent Activating Kinase: a key kinase in cell cycle control and a target for drugs? Cell Cycle; 4(4):572-7.
Harper JW, Elledge SJ. (1998) " The role of Cdk7 in CAK function, a retro-retrospective." Genes Dev. 1;12(3):285-9.
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