Recombinant human CDK9/CycT, active protein kinase,10 µg

The Cdks are serine/threonine protein kinases which are predominantly involved in the progression of the cell cycle. Some CDKs (CDK7, CDK8 and CDK9) participates as part of transcription factors in gene transcription. CDK9 is associated with T- and K-type cyclins and forms a complex with the transcription elongation factor b (P-TEFb). The complex is responsible for phosphorylation of the C-terminal domain of the largest subunit of RNA polymerase II. CDK9 is involved in controlling cell growth, cellular viability and differentiation. Additionally, CDK9 is also critical for HIV-1 and HIV-2 replication in human cells. Deregulation of the CDK9-related pathways are associated with various types of human malignancies and cardiomyocytes hypertrophy.

Human cyclin-dependent protein kinase CDK9, coexpressed in complex with with human CycT.

Active protein expressed in Sf9 insect cells and purified as fusion protein by affinity chromatography
Theoretical MW MWGST-CDK9: 72.2 kDa
Theoretical MWCycT: 85.4 kDa
Expression system: Baculovirus infected Sf9 cells
Purification: One-step affinity purification using glutathione agarose
Storage buffer: 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol
Storage temperature: - 80°C (avoid repeated freeze-thaw cycles!)
Protein concentration: 0.168 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MSFC membrane
Specific activity: 26,000 pmol/mg x min

Entrez Gene ID: 1025/904 
UniProtKB: P50750/O60563

Ordering information: shipped on dry ice

Product specific literature references:

Sano M, Schneider MD. (2003)"Cyclins that don\'t cycle--cyclin T/cyclin-dependent kinase-9 determines cardiac muscle cell size." Cell Cycle; 2(2):99-104.

Sano M, Schneider MD. (2004) "Cyclin-dependent kinase-9: an RNAPII kinase at the nexus of cardiac growth and death cascades."  Circ Res. 29;95(9):867-76.

Romano G, Giordano A. (2008) "Role of the cyclin-dependent kinase 9-related pathway in mammalian gene expression and human diseases." Cell Cycle 7(23):3664-8.