- Remove this product from my favorite's list.
- Add this product to my list of favorites.
Products
Newsletter
Cdks are the catalytic subunits of serine/threonine protein kinases which are involved in the progression through the cell cycle. The catalytic activity of these kinases requires interaction with cyclins, which are synthesized and degraded during the cell cycle in a cyclical fashion. CDK2/CycE is important for the G1/S transition and initiating DNA replication. Additionally, CDK2/CycE phosphorylates proteins involved in Histone modifications (p220, CBP/p300) and DNA repair (BRCA1) and favours by phosphorylation the degradation of p27, an inhibitor of CDK2/CycE.
Recombinant human cyclin-dependent protein kinase CDK2 amino acids M1 - L298, coexpressed with human CycE, amino acids M1 - A395
Theoretical MW GST-CDK2: 60.2 kDa
Theoretical MW GST-CycE: 72.0 kDa
Expression system: Baculovirus infected Sf9 cells
Storage buffer: 50 mM Hepes, pH 7.5; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol
Protein concentration: 0.312 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MAFC membrane
Specific activity: 88,000 pmol/mg x min
Entrez Gene ID: 1017/898
UniProtKB: P24941/P24864
Ordering information: shipped on dry ice
Gu Y, Rosenblatt J, Morgan DO (1992) "Cell cycle regulation of CDK2 activity by phosphorylation of Thr160 and Tyr15." EMBO J. 11(11):3995-4005
Cardoso MC, Leonhardt H, Nadal-Ginard B (1993) "Reversal of terminal differentiation and control of DNA replication: cyclin A and Cdk2 specifically localize at subnuclear sites of DNA replication." Cell 74(6):979-92
Gyuris J, Golemis E, Chertkov H, Brent R (1993) "Cdi1, a human G1 and S phase protein phosphatase that associates with Cdk2." Cell 75(4):791-803
Gu Y, Turck CW, Morgan DO (1993) "Inhibition of CDK2 activity in vivo by an associated 20K regulatory subunit." Nature 366(6456):707-10
Connell-Crowley L, Solomon MJ, Wei N, Harper JW (1993) "Phosphorylation independent activation of human cyclin-dependent kinase 2 by cyclin A in vitro" Mol Biol Cell. 4(1):79-92
Pagano M, Pepperkok R, Lukas J, Baldin V, Ansorge W, Bartek J, Draetta G (1993) "Regulation of the cell cycle by the cdk2 protein kinase in cultured human fibroblasts." J Cell Biol. 121(1):101-11
Jeffrey PD, Russo AA, Polyak K, Gibbs E, Hurwitz J, Massague J, Pavletich NP (1995) "Mechanism of CDK activation revealed by the structure of a cyclinA-CDK2 complex." Nature 376(6538):313-20
Radzio-Andzelm E, Lew J, Taylor S (1995) "Bound to activate: conformational consequences of cyclin binding to CDK2" Structure 3(11):1135-41
Xu X, Nakano T, Wick S, Dubay M, Brizuela L. (1999) „ Mechanism of Cdk2/Cyclin E inhibition by p27 and p27 phosphorylation.“
Sanchez I, Dynlacht BD. (2005) „New insights into cyclins, CDKs, and cell cycle control.“
Semin Cell Dev Biol.,16(3):311-21.
Kaldis P, Aleem E. (2005) „Cell cycle sibling rivalry: Cdc2 vs. Cdk2.“ Cell Cycle. 4(11):1491-4.
Malumbres M, Barbacid M. (2005) „Mammalian cyclin-dependent kinases.“ Trends Biochem Sci. 30(11):630-41.
Welcome Login
Contact us
Follow us