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Cdks are the catalytic subunits of serine/threonine protein kinases which are involved in the progression through the cell cycle. The catalytic activity of these kinases requires interaction with cyclins, which are synthesized and degraded during the cell cycle in a cyclical fashion. CDK1/CycB complexes regulate several events during both, the G2-M transition and mitosis. Active complexes undergo translocation to the nucleus and phosphorylate nuclear substrates, which are necessary for mitotic onset. Cytoplasmic CDK1/CycB is involved in regulatory and structural processes, such as chromosomal condensation and fragmentation of the Golgi network. The inactivation of CDK1/CycB complex is accomplished by degradation of cyclin B by anaphase-promoting complex, allowing mitotic exit and cell division.
Recombinant human cyclin-dependent protein kinase CDK1 (cdc2), human CDK1, amino acids M1 - M297, and human CycB1, amino acids M1-V433 both N -terminal GST-HIS6 fusion protein with a Thrombin cleavage site, coexpressed in Sf9 insect cells
Theoretical MW GST-CDK1: 63.882 kDa
Theoretical MW GST-CycB1: 78.925 kDa
Expression system: Baculovirus infected Sf9 cells
Storage buffer: 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol
Protein concentration: 0.255 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MSFC membrane
Specific activity: 7,600 pmol/mg x min
Activation: This kinase was not activated by special procedures
Entrez Gene ID: 983/891 UniProtKB: P06493/ P14635
Ordering information: shipped on dry ice
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Recombinant human CDK2/CycA2, active protein kinase,10 µg - 465,00 €
Recombinant human CDK6/CycD1, active protein kinase, 10 µg - 465,00 €
Recombinant human CDK4/CycD1, active protein kinase,10 µg - 465,00 €
CDC2 substrate, 1 mg - 150,00 €
Recombinant human CDK1/CycA, active protein kinase,10 µg - 465,00 €
Recombinant human CDK5/p25NCK, active protein kinase,10µg - 465,00 €
Recombinant human CDK8/CycC, active protein kinase, 10 µg - 465,00 €
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