Recombinant Mouse PD-L1 /CD274 /B7-H1 Protein, With C-Fc Tag, 200µg

Background
Programmed cell death 1 ligand 1 (PD-L1) is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. B7-H1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. PD-L1 is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 involve in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner.

Source
Recombinant Mouse PD-L1 /CD274 /B7-H1 Protein, With C-Fc Tag (Mouse PDL1 Fc Chimera) Phe 19 - Thr 238 (Accession # NP_068693) was produced in human 293 cells (HEK293).

Molecular Characterization
Mouse PDL1 Fc Chimera is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 51.4 kDa. The predicted N-terminus is Phe 19. DTT-reduced Protein migrates as 65-85 kDa in SDS-PAGE due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the Mouse PDL1 Fc Chimera by the LAL method.

Purity
>92% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized Mouse PD-L1 / B7-H1 Protein, FcTag ( Cat# PD1-M5251,1 μg / well) can bind Biotinylated Mouse PD-1 / PDCD1, Fc Tag, Avi Tag (Avitag™) (Cat# PD1-M82F4). The linear range is 0.15-2.5 μg/mL.

References

(1) Dong H., et al., 1999, Nat. Med. 5:1365-1369.
(2) Freeman G.J., et al., 2000, J. Exp. Med. 192:1027-1034.
(3) Nishimura H., et al., 2001, Trends in Immunology 22:265.