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Recombinant human 4-1BB Ligand / TNFSF9 Protein, human Fc Tag, 50µg  

Recombinant human 4-1BB Ligand / TNFSF9 Protein, human Fc Tag, 50µg

Recombinant Human 4-1BB Ligand, Ala 50 - Glu 254, expressed from human 293 cells (HEK293), Fc Tag

Synonym: recombinant human protein 4-1BB Ligand, TNFSF9, CD137L

More details

41L-H5257-050

Availability: within 7 days

420,00 €

Background
Tumor necrosis factor ligand superfamily member 9 (4-1BBL) is also known as 4-1BB ligand, CD137L or TNFSF9, which is a cytokine that binds to TNFRSF9. 4-1BBL is the high affinity ligand of 4-1BB. 4-1BBL induces the proliferation of activated peripheral blood T-cells. Also, 4-1BBL may have a role in activation-induced cell death (AICD). Furthermore, 4-1BBL may play a role in cognate interactions between T-cells and B-cells/macrophages. As for diseases, 4-1BBL is involved in cancers, infectious diseases and autoimmune diseases.

Source
Recombinant Human 4-1BB Ligand, Fc Tag (41L-H5257) is expressed from human 293 cells (HEK293). It contains AA Ala 50 - Glu 254 (Accession # NP_003802).
Predicted N-terminus: Ala 50

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.The protein has a calculated MW of 47.5 kDa. The protein migrates as 52 kDa under reducing (R) condition (SDS-PAGE).

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
Please refer to product data sheet.

References

(1) Won E.Y., et al., 2010, J. Biol. Chem. 285:9202-9210.
(2) Alderson M.R., et al., 1994, Eur. J. Immunol. 24:2219-2227.