Recombinant human CD27 / TNFRSF7 Protein, human Fc Tag, 200µg

Background
CD Antigen CD27 also known as Tumor necrosis factor receptor superfamily member 7 (TNFRSF7), which belongs to TNF-receptor superfamily. CD27 / TNFRSF7 is found in most T-lymphocytes. CD27 / TNFRSF7 is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-κB and MAPK8/JNK. CD27 has been shown to interact with SIVA1, TRAF2 and TRAF3.

Source
Recombinant Human CD27 /TNFRSF7 Protein, Fc Tag (CD7-H5254) is expressed from human 293 cells (HEK293). It contains AA Thr 21 - Ile 192 (Accession # NP_001233).
Predicted N-terminus: Thr 21

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 45.9 kDa. The protein migrates as 53-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>85% as determined by SEC-HPLC.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Bioactivity
Please refer to product data sheet.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "Soluble immune checkpoint protein CD27 is a novel prognostic biomarker of HCC development in HCV-SVR patients"
Dong, Thanh Thuy, Hoang et al
Am J Pathol (2022)
(2) "Early detection of soluble CD27, BTLA, and TIM-3 predicts the development of nosocomial infection in pediatric burn patients"
Penatzer, Alexander, Simon et al
Front Immunol (2022) 13, 940835
(3) "Variant in the PLCG2 Gene May Cause a Phenotypic Overlap of APLAID/PLAID: Case Series and Literature Review"
Welzel, Oefelein, Holzer et al
J Clin Med (2022) 11 (15)
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