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Recombinant Human TIM-3 / HAVCR2 Protein, 100µg  

Recombinant Human TIM-3 / HAVCR2 Protein, 100µg

Recombinant Human TIM3 /HAVCR2 Protein, His Tag (rhTIM3 / HAVCR2) Ser 22 - Arg 200 was produced in human 293 cells (HEK293) (MALS verified)

Synonym: recombinant, human, protein, HAVCR2, HAVcr-2, KIM3, TIM3, TIMD-3, TIMD3, Tim-3, FLJ14428, KIM-3, KIM3

More details

TM3-H5229-100

Availability: within 7 days

403,00 €

Background
Hepatitis A virus cellular receptor 2 is also known as HAVCR2, FLJ14428, KIM3, TIM3, TIMD3, is a member of the TIM family of immune regulating molecules with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells and their associated cytokines are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. The 2 types of cells also cross-regulate the functions of the other. HAVCR2 is a Th1-specific cell surface protein that regulates macrophage activation and enhances the severity of experimental autoimmune encephalomyelitis in mice. HAVCR2 regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Dysregulation of the HAVCR2-galectin-9 pathway could underlie chronic autoimmune disease states in human, such as multiple sclerosis.

Source
Recombinant Human TIM-3, His Tag (TM3-H5229) is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Arg 200 (Accession # Q8TDQ0-1).
Predicted N-terminus: Ser 22

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 21.8 kDa. The protein migrates as 44-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Bioactivity
Please refer to product data sheet.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "Cholesterol induction in CD8+ T cell exhaustion in colorectal cancer via the regulation of endoplasmic reticulum-mitochondria contact sites"
Shuwen, Yinhang, Jing et al
Cancer Immunol Immunother (2023)
(2) "Soluble immune checkpoints are elevated in patients with primary biliary cholangitis"
Gao, Wang, Guan et al
Eur J Med Res (2023) 28 (1), 477
(3) "Cutting Edge: Serpine1 Negatively Regulates Th1 Cell Responses in Experimental Autoimmune Encephalomyelitis"
Akbar, Tang, Baillargeon et al
J Immunol (2023)
Showing 1-3 of 2298 papers.