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Recombinant Human GITR Ligand / TNFSF18 Protein, 50µg  

Recombinant Human GITR Ligand / TNFSF18 Protein, 50µg

Recombinant Human GITR Ligand, Fc Tag (GIL-H526a) is expressed from human 293 cells (HEK293).

Synonym: recombinant, human, protein,TNFSF18,AITRL,TL6,hGITRL,GITR Ligand

More details

GIL-H526a-050

Availability: within 7 days

420,00 €

Background
Tumor necrosis factor ligand superfamily member 18 (TNFSF18) is also known as Glucocorticoid - induced TNF-related ligand (GITRL or GITR Ligand), Activation - inducible TNF - related ligand (AITRL), which belongs to the tumor necrosis factor family. TNFSF18 is expressed at high levels in the small intestine, ovary, testis, kidney and endothelial cells. TNFSF18 / GITRL is up-regulated after stimulation by bacterial lipopolysaccharides (LPS). TNFSF18 Can function as costimulator and lower the threshold for T-cell activation and T-cell proliferation. TNFSF18 / GITR Ligand is important for interactions between activated T-lymphocytes and endothelial cells. TNFSF18 also mediates activation of NF-kappa-B.

Source
Recombinant Human GITR Ligand, Fc Tag (GIL-H526a) is expressed from human 293 cells (HEK293). It contains AA Gln 50 - Ser 177 (Accession # AAH69319.1).

Molecular Characterization
This protein carries a human IgG1 Fc tag at the N-terminus.
The protein has a calculated MW of 41.5 kDa. The protein migrates as 45-48 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rh GITR Ligand / TNFSF18 Fc Chimera by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Bioactivity
Please refer to product data sheet.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

References

(1) Tuyaerts S., et al., 2007, J. Leukoc. Biol. 82:93-105.
(2) Chattopadhyay K., et al., 2007, Proc. Natl. Acad. Sci. U.S.A. 104:19452-19457.