Recombinant human HVEM / TNFRSF14 Protein, Strep Tag, 25µg

Background
Herpesvirus entry mediator (HVEM) is also known as TNFRSF14, TR2 (TNF receptorlike molecule) and ATAR (another TRAF associated receptor), is a type I membrane protein belonging to the TNF/NGF receptor superfamily. HVEM expression has been detected in peripheral blood T cells, B cells, monocytes and in various tissues enriched in lymphoid cells. The extracellular domain of HVEM has been shown to interact directly with the herpes simplex virus envelope glycoprotein D (gD). Two TNF superfamily ligands, including the secreted TNFβ (lymphotoxin α) and the membrane protein LIGHT (lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes), have been shown to be the cellular ligands for HVEM. Besides HVEM, LIGHT can also interact with LTβR, the receptor for lymphotoxin αβ heterotrimer. The role of the HVEM LIGHT /LTβ receptor ligand pair in immune function and herpesvirus pathobiology remains to be elucidated.

Source
Recombinant Human HVEM Protein, With C-Twin Strep Tag (rh HVEM,twin strep tag) Leu 39 - Val 202 (Accession # NP_003811.2) was produced in human 293 cells (HEK293).

Molecular Characterization
rh HVEM,twin strep tag is fused with a Twin-Strep tag at the C-terminus, and has a calculated MW of 20.4 kDa. The predicted N-terminus is Leu 39. DTT-reduced Protein migrates as 27-50 kDa in SDS-PAGE due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rh HVEM,twin strep tag by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Bioactivity
n/a

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

References

(1) Hsu H., et al., 1997, J. Biol. Chem., 272:13471.
(2) Mauri, D.N. et al., 1998, Immunity 8:21.
(3) Montgomery, R.I. et al., 1996, Cell 87:427.
(4) Cheung,T.C.et al., 2005, Proc.Natl.Acad.Sci.USA102:13218-23.