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Recombinant Mouse CD28 Protein, Fc Tag, 2x 500µg  

Recombinant Mouse CD28 Protein, Fc Tag, 2x 500µg

Recombinant Mouse CD28 Protein, C-Fc Tag (Mouse CD28 Fc Chimera) Asn 20 - Lys 149  was produced in human 293 cells (HEK293).

Synonym: recombinant, mouse, protein, CD28,Tp44

More details

CD8-M5257-1K

Availability: within 7 days

1 200,00 €

Background
T-cell-specific surface glycoprotein CD28 is also known as TP44, is a single-pass type I membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.

Source
Recombinant Mouse CD28 Protein, C-Fc Tag (Mouse CD28 Fc Chimera) Asn 20 - Lys 149 (Accession # NP_031668.3) was produced in human 293 cells (HEK293).

Molecular Characterization
Mouse CD28 Fc Chimera is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 41.7 kDa. The predicted N-terminus is Asn 20. DTT-reduced Protein migrates as 55-60 kDa in SDS-PAGE due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the Mouse CD28 Fc Chimera by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
Immobilized Mouse CD28, Fc Tag (Cat. No. CD8-M5257) at 5 μg/mL (100 μL/well)can bind Biotinylated Human B7-2, Fc Tag (Cat. No. CD6-H82F5) with a linear range of 0.156-2.5 μg/mL (QC tested).

References

(1) Woerly G., et al., 2002, J Leukoc Biol 72 (4): 769–79.
(2) Nunès, J A., et al., 1996, J. Biol. Chem. (UNITED STATES) 271 (3): 1591–8.
(3) Ellis, J H., et al., 2000, J. Immunol. (UNITED STATES) 164 (11): 5805–14.