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Recombinant human B7-H4 Protein, 1mg  

Recombinant human B7-H4 Protein, 1mg

Recombinant Human B7-H4 /B7S1 /VTCN1 Protein, Phe 29 - Ala 258, produced in human 293 cells (HEK293), His Tag

Synonym: recombinant, human, protein B7-H4, VTCN1

More details

B74-H5222-1K

Availability: within 7 days

1 500,00 €

Background
V-set domain-containing T-cell activation inhibitor 1 (VTCN1) is also known as Immune costimulatory protein B7-H4, Protein B7S1, T-cell costimulatory molecule B7x, B7H4, which belongs to the immunoglobulin superfamily and BTN/MOG family. VTCN1 contains two Ig-like V-type (immunoglobulin-like) domains. The expression of VTCN1 is up-regulated by IL6 and IL10 and is inhibited by GM-CSF and IL4 on antigen-presenting cells (APCs). VTCN1 / B7-H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. VTCN1 involved in promoting epithelial cell transformation.

Source
Recombinant Human B7-H4, His Tag (B74-H5222) is expressed from human 293 cells (HEK293). It contains AA Phe 29 - Ala 258 (Accession # NP_078902).
Predicted N-terminus: Phe 29

Molecular Characterization
rh B7-H4 / B7S1 / VTCN1, fused with polyhistidine tag at the C-terminus, has a calculated MW of 26.1 kDa. The predicted N-terminus is Phe 29. DTT-reduced Protein migrates as 43-60 kDa due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rh B7-H4 / B7S1 / VTCN1 by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
Immobilized Human B7-H4, His Tag (Cat. No. B74-H5222) at 2 μg/mL (100 μL/well) can bind Anti-B7-H4 MAb (Human IgG1) with a linear range of 0.2-6 ng/mL (QC tested).

References

(1) Salceda S., et al., 2005, Exp. Cell Res. 306:128-141.
(2) Kryczek I., et al., 2006, J. Exp. Med. 203:871-881.
(3) Miyatake T., et al., 2007, Gynecol. Oncol. 106:119-127.