Products

Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant Mouse CD28 Protein, His-Tag, 2x 500 µg  

Recombinant Mouse CD28 Protein, His-Tag, 2x 500 µg

Recombinant Mouse CD28 Protein (Mouse CD28), Asn 20 - Lys 149, produced in human 293 cells (HEK293), His tag

Synonym: recombinant, mouse, protein, CD28,Tp44

More details

CD8-M52H6-1K

Availability: within 7 days

1 800,00 €

Background
T-cell-specific surface glycoprotein CD28 is also known as TP44, is a single-pass type I membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.

Source
Mouse CD28, His Tag (CD8-M52H6) is expressed from human 293 cells (HEK293). It contains AA Asn 20 - Lys 149 (Accession # P31041-1).
Predicted N-terminus: Asn 20

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 16.9 kDa. The protein migrates as 30-35 kDa and 55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the Mouse CD28 by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Bioactivity
Immobilized Mouse CD28, His Tag (Cat. No. CD8-M52H6) at 5 μg/mL (100 μL/well) can bind Mouse B7-1, Fc Tag (Cat. No. CD0-M5259) with a linear range of 0.156-5 μg/mL (QC tested).

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

References

(1) Woerly G., et al., 2002, J Leukoc Biol 72 (4): 769–79.
(2) Nunès, J A., et al., 1996, J. Biol. Chem. (UNITED STATES) 271 (3): 1591–8.
(3) Ellis, J H., et al., 2000, J. Immunol. (UNITED STATES) 164 (11): 5805–14.