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Recombinant Mouse CD28 Protein, His Tag, active dimer (MALS verified), 2x 500 µg  

Recombinant Mouse CD28 Protein, His Tag, active dimer (MALS verified), 2x 500 µg

Recombinant Mouse CD28 Protein (Mouse CD28), Asn 20 - Lys 149, produced in human 293 cells (HEK293), His Tag, active dimer (MALS verified)

Synonym: recombinant, mouse, protein, CD28,Tp44

More details

CD8-M52H6-1K

Availability: within 7 days

1 800,00 €

Background
T-cell-specific surface glycoprotein CD28 is also known as TP44, is a single-pass type I membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.

Source
Recombinant Mouse CD28, His Tag (CD8-M52H6) is expressed from human 293 cells (HEK293). It contains AA Asn 20 - Lys 149 (Accession # P31041-1).
Predicted N-terminus: Asn 20

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 16.9 kDa. As a result of glycosylation, the protein migrates as 33-40 kDa under reducing (R) condition, and 55-66 kDa under non-reducing (NR) condition (SDS-PAGE).

Endotoxin
Less than 1.0 EU per μg of the Mouse CD28 by the LAL method.

Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Bioactivity
Please refer to product data sheet.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

References

(1) "[Blockade of D1-like dopaminergic receptors suppresses Th17-cell function in multiple sclerosis]"
Melnikov, Sviridova, Solodova et al
Zh Nevrol Psikhiatr Im S S Korsakova (2021) 121 (7. Vyp. 2), 82-89
(2) "Modulation of Xenogeneic T Cell Proliferation by B7 and mTOR Blockade of T cells and Porcine Endothelial Cells"
Li, Xu, Kirk
Transplantation (2021)
(3) "Expression of programmed death ligand 1 in drug-resistant osteosarcoma: An exploratory study"
Skertich, Chu, Tarhoni et al
Surg Open Sci (2021) 6, 10-14

Showing 1-3 of 12142 papers.
Please refer to product data sheet.