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Recombinant Human B7-H5 / Gi24 / VISTA Protein, His-Tag, 100µg  

Recombinant Human B7-H5 / Gi24 / VISTA Protein, His-Tag, 100µg

Recombinant Human B7-H5 / Gi24 / VISTA Protein (rh B7-H5 / Gi24 / VISTA) Phe 33 - Ala 194 was produced in human 293 cells (HEK293) fused with  a polyhistidine tag, His Tag.

Synonym: recombinant, human, protein B7-H5,SISP1,Gi24,VISTA

More details

B75-H52H0-100

Availability: within 7 days

336,00 €

Background
Platelet receptor Gi24, also known as B7-H5 and stress-induced secreted protein-1 (Sisp-1), is a protein that in humans is encoded by the C10orf54 gene, which contains 1 Ig-like (immunoglobulin-like) domain. As for C10orf54 gene, C10orf54 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state on ESCs. Human C10orf54 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment..

Source
Recombinant Human B7-H5 /Gi24/VISTA Protein (rh B7-H5 / Gi24 / VISTA) Phe 33 - Ala 194 (Accession # AAH20568) was produced in human 293 cells (HEK293).

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 20.0 kDa. The protein migrates as 33-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rh B7-H5 / Gi24 / VISTA by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

References

(1) Sakr M.A., et al., 2010, Cancer Sci. 101:2368-2374.
(2) Mayya V., et al ., 2009, Sci. Signal. 2:RA46-RA46.