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Recombinant Mouse CD40 / TNFRSF5 Protein, His-Tag 1mg  

Recombinant Mouse CD40 / TNFRSF5 Protein, His-Tag 1mg

Recombinant Mouse CD40 / TNFRSF5 Protein (Mouse CD40, His Tag) Val 24 - Arg 193 was produced in human 293 cells (HEK293).

Synonym: recombinant mouse protein CD40,Bp50,TNFRSF5,p50

More details

TN5-M52H8-1K

Availability: within 7 days

1 800,00 €

Background
CD40 is also known as TNFRSF5, Bp50, CDW40, MGC9013, TNFRSF5 and p50, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins, and plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects. CD40 contains 4 cysteine-rich repeats in the extracellular domain, and is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines. The extracellular domain has the cysteinerich repeat regions, which are characteristic for many of the receptors of the TNF superfamily. Interaction of CD40 with its ligand, CD40L, leads to aggregation of CD40 molecules,which in turn interact with cytoplasmic components to initiate signaling pathways. Early studies on the CD40-CD40L system revealed its role in humoral immunity. Defects in CD40 result in hyper-IgM immunodeficiency type 3 (HIGM3), an autosomal recessive disorder characterized by an inability of B cells to undergo isotype switching, as well as an inability to mount an antibody-specific immune response, and a lack of germinal center formation.

Source
Recombinant Mouse CD40 / TNFRSF5 Protein, His Tag (Mouse CD40, His Tag) Val 24 - Arg 193 (Accession # P27512) was produced in human 293 cells (HEK293).

Molecular Characterization
Mouse CD40, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 20.8 kDa. The predicted N-terminus is Val 24. DTT-reduced Protein migrates as 30 kDa in SDS-PAGE due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the Mouse CD40, His Tag by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
Immobilized Mouse CD40, His Tag (Catalog # TN5-M52H8) at 5μg/mL (100 μL/well) can bind Mouse CD40 Ligand, Fc Tag (Catalog # CDL-M526x) with a linear range of 1-16 ng/mL.

References

(1) Banchereau, J. et al., 1994, Annu. Rev. Immunol. 12: 881-922.
(2) Ni, C.Z. et al., 2000, Proc. Natl. Acad. Sci. U.S.A. 97: 10395-10399.
(3) Ferrari.S. et al., 2001, Proc. Natl. Acad. Sci. U.S.A. 98: 12614-12619.
(4) Bhushan, A and Covey, L.R. 2001, Immunol. Res. 24: 311-324.
(5) Armitage, R.J. et al., 1993, J Immunol., 150 (9): 3671-3680.

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