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Recombinant Cynomolgus / Rhesus macaque 4-1BB / TNFRSF9 Protein, 2x 500 µg  

Recombinant Cynomolgus / Rhesus macaque 4-1BB / TNFRSF9 Protein, 2x 500 µg

Recombinant Cynomolgus / Rhesus macaque 4-1BB, His Tag (41B-C52H4) is expressed from human 293 cells (HEK293)

Synonym: recombinant cynomolgus, protein TNFRSF9,4-1BB,CD137,CDw137,ILA

More details

41B-C52H4-1K

Availability: within 7 days

1 980,00 €

Background
4-1BB is also known as CD137, tumor necrosis factor receptor superfamily member 9 (TNFRSF9), induced by lymphocyte activation (ILA), is a co-stimulatory molecule of the tumor necrosis factor (TNF) receptor superfamily. CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. In addition, CD137 expression is found on dendritic cells, follicular dendritic cells, natural killer cells, granulocytes and cells of blood vessel walls at sites of inflammation. The best characterized activity of CD137 is its costimulatory activity for activated T cells. Crosslinking of CD137 enhances T cell proliferation, IL-2 secretion survival and cytolytic activity. Further, it can enhance immune activity to eliminate tumors in mice. CD137 can enhance activation-induced T cell apoptosis when triggered by engagement of the TCR/CD3 complex. In addition, 4-1BB/4-1BBL co-stimulatory pathway has been shown to augment secondary CTL responses to several viruses, and meanwhile augment anti-tumor immunity. 4-1BB thus is a promising candidate for immunotherapy of human cancer. CD137 has been shown to interact with TRAF2.

Source
Cynomolgus / Rhesus macaque 4-1BB, His Tag (41B-C52H4) is expressed from human 293 cells (HEK293). It contains AA Leu 24 - Gln 186 (Accession # XP_005544945.1).
Predicted N-terminus: Leu 24

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 19.2 kDa. The protein migrates as 30-40 kDa under reducing (R) condition (SDS-PAGE).

Endotoxin
Less than 1.0 EU per μg of the Cynomolgus 4-1BB, His Tag by the LAL method.

Purity
>92% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized Cynomolgus 4-1BB, His Tag (Cat# 41B-C52H4) at 1μg/mL (100 µl/well),can bind Human 4-1BB Ligand, Fc Tag (Cat# 41L-H5257) with a linear of 0.01-0.5 ng/mL. 

References

(1) Cooper D, et al., 2002, Eur. J. Immunol. 32 (2): 521–9.
(2) Jang, I K., et al., 1998, Biochem. Biophys. Res. Commun. (UNITED STATES) 242 (3): 613–20.
(3) Arch, R H., Thompson C B., 1998, Mol. Cell. Biol. (UNITED STATES) 18 (1): 558–65.