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Recombinant Human PD-L2 / B7-DC Protein, mouse IgG1 Fc Tag, 100µg  

Recombinant Human PD-L2 / B7-DC Protein, mouse IgG1 Fc Tag, 100µg

Recombinant Human PD-L2, Leu 20 - Pro 219, expressed from human 293 cells (HEK293), mouse IgG1 Fc Tag

Synonym: human, recombinant, protein, PDL2,PD-L2,Butyrophilin B7-DC,CD273,PDCD1 ligand 2

More details

PD2-H52A5-100

Availability: within 7 days

348,00 €

Background
Programmed cell death 1 ligand 2 (PD-L2 or PDCD1 ligand 2) is also known as Butyrophilin B7-DC, CD antigen CD273, which belongs to the immunoglobulin superfamily or BTN/MOG family. The expression of PD-L2 is up-regulated by IFNG/IFN-gamma stimulation in monocytes and induced on dendritic cells grown from peripheral blood mononuclear cells with CSF2 and IL-4. PD-L2 Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. PD-L2 interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.

Source
Human PD-L2, mouse IgG1 Fc Tag (PD2-H52A5) is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Pro 219 (Accession # AAI13679).
Predicted N-terminus: Leu 20
Request for sequence

Molecular Characterization
This protein carries a mouse IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 48.9 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the Human PD-L2, Mouse Fc Tag by the LAL method.

Purity
>95% as determined by reduced SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity-ELISA
Immobilized Human PD-1, His Tag (Cat. No. PD1-H5221) at 2 μg/mL (100 μL/well) can bind Human PD-L2, Mouse IgG1 Fc Tag (Cat. No. PD2-H52A5) with a linear range of 10-156 ng/mL (QC tested).

Serial dilutions of nivolumab were added into Human B7-1, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. B71-H52A4): Biotinylated Human PD-1, Fc,Avitag (Cat. No. PD1-H82F1) binding reactions. The half maximal inhibitory concentration (IC50) is 0.2823 μg/mL (Routinely tested).

Bioactivity-FACS
Flow Cytometry assay shows that Human PD-L2, Mouse IgG1 Fc Tag (Cat. No. PD1-H52A3) can bind to 293 cell overexpressing human PD-1. The concentration of PD-L2 used is 0.1 μg/mL (Routinely tested).

FACS analysis shows that the binding of Human PD-L2, Mouse IgG1 Fc Tag (Cat. No. PD1-H52A3) to 293 overexpressing PD-1 was inhibited by increasing concentration of neutralizing Anti-PD-1 antibody. The concentration of PD-L2 used is 0.1 μg/mL. The IC50 is 1.3 μg/mL (Routinely tested).

References

(1) Tseng S.-Y., et al., 2001, J. Exp. Med. 193:839-846.
(2) Latchman Y., et al., 2001, Nat. Immunol. 2:261-268.
(3) He X.-H., et al., 2004, Acta Biochim. Biophys. Sin. 36:284-289.