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Recombinant Mouse TIM-3 / HAVCR2 Protein, Fc Tag , 1mg  

Recombinant Mouse TIM-3 / HAVCR2 Protein, Fc Tag , 1mg

Recombinant Mouse TIM-3 / HAVCR2 Protein, Fc Tag (Mouse TIM-3, Fc Tag) Arg 20 - Arg 191 was produced in human 293 cells (HEK293).

Synonym: recombinant, mouse, protein, HAVCR2,TIM3,TIMD3,FLJ14428,KIM3

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TI3-M5252-1K

Availability: within 7 days

1 800,00 €

Background
Hepatitis A virus cellular receptor 2 is also known as HAVCR2, FLJ14428, KIM3, TIM3, TIMD3, is a member of the TIM family of immune regulating molecules with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells and their associated cytokines are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. The 2 types of cells also cross-regulate the functions of the other. HAVCR2 is a Th1-specific cell surface protein that regulates macrophage activation and enhances the severity of experimental autoimmune encephalomyelitis in mice. HAVCR2 regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Dysregulation of the HAVCR2-galectin-9 pathway could underlie chronic autoimmune disease states in human, such as multiple sclerosis.

Source
Recombinant Mouse TIM-3 / HAVCR2 Protein, Fc Tag (Mouse TIM-3, Fc Tag) Arg 20 - Arg 191 (Accession # AAL65156.1) was produced in human 293 cells (HEK293).

Molecular Characterization
Mouse TIM-3, Fc Tag is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 45.8 kDa. The predicted N-terminus is Arg 20. DTT-reduced Protein migrates as 60-80 kDa in SDS-PAGE.

Endotoxin
Less than 1.0 EU per μg of the Mouse TIM-3, Fc Tag by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

References

(1) Monney, L. et al., 2002, Nature. 415: 536-41.
(2) Sabatos, CA. et al., 2003, Nat. Immunol. 4: 1102-10.
(3) Anderson, AC. et al., 2006, Curr. Opin. Immunol. 18: 665-9.