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Recombinant Biotinylated Human VEGF121, Avi Tag (Avitag™), 200µg  

Recombinant Biotinylated Human VEGF121, Avi Tag (Avitag™), 200µg

Recombinant Biotinylated Human VEGF121Protein, His Tag (VE1-H82E1) is expressed from human HEK293 cells, It contains AA Ala 27 - Arg 147, Avitag™

Synonym
recombinant, human, biotinylated, protein VEGF121 Protein, His Tag, RP1-261G23.1, MGC70609, MVCD1, VEGFA, VPF

More details

VE1-H82E7-200

Availability: within 7 days

1 380,00 €

Background
Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) and VEGF-A, and is a member of the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. Alternatively spliced isoforms of 121,145,165,183,189 and 206 amino acids in length are expressed in humans. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189. VEGF121 is the only form that lacks a basic heparinbinding region and is freely diffusible. Mouse embryos expressing only the corresponding isoform (VEGF120) do not survive to term, and show defects in skeletogenesis. Human VEGF121 shares 87% aa sequence identity with corresponding regions of mouse and rat, 93% with feline, equine and bovine, and 91%, 95% and 96% with ovine, canine and porcine VEGF, respectively. VEGF121 induces the proliferation of lymphatic endothelial cells. The lymphangiogenesis may be promoted by upregulation of VEGF121, which may in turn act in part via induction of VEGF-C.

Source
MABSol® Recombinant Biotinylated Human VEGF121, Avitag, His Tag (VE1-H82E1) is expressed from human HEK293 cells. It contains AA Ala 27 - Arg 147 (Accession # P15692-9).

Molecular Characterization
This protein carries an Avi tag at the N-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 16.7 kDa. As a result of glycosylation, the protein migrates as 18 kDa and 22 kDa under reducing (R) condition, and 35-40 kDa under non-reducing (NR) condition (SDS-PAGE).

Biotinylation
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Purity
>95% as determined by reduced SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the COA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Limited Use License
The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrosbiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.
Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays. 

Clinical and Translational Updates

(1) "Isoforms of soluble vascular endothelial growth factor in stress-related mental disorders: a cross-sectional study"
Wallensten, Mobarrez, Åsberg et al
Sci Rep (2021) 11 (1), 16693
(2) "Investigation of the mechanisms of VEGF-mediated compensatory lung growth: the role of the VEGF heparin-binding domain"
Yu, Ko, Dao et al
Sci Rep (2021) 11 (1), 11827
(3) "Proteomic Characterization of Human Neural Stem Cells and Their Secretome During in vitro Differentiation"
Červenka, Tylečková, Kupcová Skalníková et al
Front Cell Neurosci (2020) 14, 612560
Showing 1-3 of 424 papers.
Please refer to product data sheet.