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Recombinant Mouse CD40 Ligand / TNFSF5 Protein, 100µg  

Recombinant Mouse CD40 Ligand / TNFSF5 Protein, 100µg

Recombinant Mouse CD40 Ligand / TNFSF5 Protein, Gly 115 - Leu 260, expressed in human 293 cells (HEK293), His Tag (active trimer) (MALS verified)

Synonym
recombinant, mouse, protein, CD40LG,CD154,CD40L,HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39

More details

CDL-M5248-100

Availability: within 7 days

348,00 €

Background
CD40 ligand is also known as CD40L, CD154, TNFSF5 and T-cell antigen Gp39, is a single-pass type I I membrane protein which belongs to the TNF superfamily of molecules. CD40 ligand is expressed predominantly on activated CD4+ T lymphocytes, and also found in other types of cells, including platelets, mast cells, macrophages, basophils, NK cells, B lymphocytes, as well as non-haematopoietic cells (smooth muscle cells, endothelial cells, and epithelial cells). Although all monomeric, dimeric and trimeric forms of soluble CD40 ligand can bind to CD40, the trimeric form of soluble CD40 ligand has the most potent biological activity through oligomerization of cell surface CD40, a common feature of TNF receptor family members. CD40 ligand binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In general, CD40 ligand plays the role of a costimulatory molecule and induces activation in APC in association with T cell receptor stimulation by MHC molecules on the APC. In total CD40 ligand has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD40 ligand regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper IgM syndrome.

Source
Recombinant mouse CD40 Ligand / TNFSF5 Protein (Mouse CD40 Ligand), His Tag (active trimer) (MALS verified) (CDL-M5248) is expressed from human 293 cells (HEK293). It contains AA Gly 115 - Leu 260 (Accession # P27548-1).
Predicted N-terminus: His

Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 16.8 kDa. The protein migrates as 19-22 kDa under reducing (R) condition (SDS-PAGE).

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). 

Bioactivity
Please refer to product data sheet.

References

Please refer to product data sheet.