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Recombinant Human CD155 / PVR Protein, Fc Tag, 4 x 250µg  

Recombinant Human CD155 / PVR Protein, Fc Tag, 4 x 250µg

Recombinant Human CD155 / PVR Protein, Trp 21 - Asn 343, expressed in human 293 cells (HEK293), Fc Tag

Synonym
recombinant, human, protein, PVR,FLJ25946,PVS,CD155,TAGE4,HVED,NECL5

More details

CD5-H5251-1K

Availability: within 7 days

1 800,00 €

Background
CD155 is a Type I transmembrane glycoprotein in the immunoglobulin superfamily. Commonly known as Poliovirus Receptor (PVR) due to its involvement in the cellular poliovirus infection in primates, CD155's normal cellular function is in the establishment of intercellular adherens junctions between epithelial cells. CD155/PVR was originally isolated based on its ability to mediate polio virus attachment to host cells. The fulllength (or CD155 alpha isoform) is synthesized as a 417 amino acid (aa) precursor that contains a 20 aa signal sequence, a 323 aa extracellular region, a 24 aa TM segment and a 50 aa cytoplasmic tail. The extracellular region contains one N terminal V type and two C2 type Ig like domains. CD155 is a transmembrane protein with 3 extracellular immunoglobulin-like domains, D1-D3, where D1 is recognized by the virus. Low resolution structures of CD155 complexed with poliovirus have been obtained using electron microscopy while a high resolution structures of theectodomain D1 and D2 of CD155 were solved by x-ray crystallography.

Source
Recombinant Human CD155, Fc Tag (CD5-H5251) is expressed from human 293 cells (HEK293). It contains AA Trp 21 - Asn 343 (Accession # NP_006496.2).
Predicted N-terminus: Trp 21

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 61.7 kDa. The protein migrates as 95-105 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer tp product data sheet.

References

(1) Mendelsohn CL, Wimmer E, Racaniello VR, 1989, Cell 56 (5): 855–65 .
(2) Maier MK, Seth S, Czeloth N, et al., 2007, European Journal of Immunology 37 (8): 2214.
(3) Koike, H. et al., 1990, EMBO J. 9:3217.
(4) Zhang P, Mueller S, Morais MC. Proc. Natl. Acad. Sci. U.S.A. 105 (47): 18284–9.