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Recombinant Cynomolgus / Rhesus macaque TIGIT Protein, 2x500µg  

Recombinant Cynomolgus / Rhesus macaque TIGIT Protein, 2x500µg

Recombinant Cynomolgus / Rhesus macaque TIGIT, His Tag (TIT-C5223) is expressed from human 293 cells (HEK293). It contains AA Met 89 - Ile 208. Predicted N-terminus: Met 89

Synonym: recombinant, human, protein, TIGIT, VSIG9, VSTM3

More details

TIT-C5223-1K

Availability: within 7 days

2 180,00 €

Background
T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is also known as V-set and immunoglobulin domain-containing protein 9 (VSIG9), V-set and transmembrane domain-containing protein 3 (VSTM3),which belongs to single-pass type I membrane protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level). TIGIT binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.

Source
Recombinant Cynomolgus / Rhesus macaque TIGIT, His Tag (TIT-C5223) is expressed from human 293 cells (HEK293). It contains AA Met 89 - Ile 208 (Accession # G7NXM4). Predicted N-terminus: Met 89

Molecular Characterization
Cynomolgus TIGIT, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 15.2 kDa. The predicted N-terminus is Met 89. The reducing (R) protein migrates as 20-23 kDa in SDS-PAGE.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized Cynomolgus TIGIT, His Tag (Catalog # TIT-C5223) at 5 μg/mL (100 μL/well) can bind Human CD155, Fc Tag (Catalog # CD5-H5251) with a linear range of 0.078-2.5 μg/mL.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

References

(1) Yu X., et al., 2009, Nat. Immunol. 10:48-57.
(2) Stengel K.F., et al., 2012, Proc. Natl. Acad. Sci. U.S.A. 109:5399-5404.
(3) Lozano E., et al., 2012, J. Immunol. 188:3869-3875.