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Recombinant Human CD155 / PVR Protein, Mouse IgG2a Fc Tag, low endotoxin , 1mg  

Recombinant Human CD155 / PVR Protein, Mouse IgG2a Fc Tag, low endotoxin , 1mg

Recombinant Human CD155 / PVR, Trp 21 - Asn 343, expressed from human 293 cells (HEK293), mouse IgG2a Fc Tag,

Synonym
recombinant, human, protein, PVR, FLJ25946, PVS, CD155, TAGE4, HVED, NECL5

More details

CD5-H5254-1K

Availability: within 7 days

2 100,00 €

Background
CD155 (cluster of differentiation 155) also known as the poliovirus receptor is a protein that is encoded by the PVR gene. CD155 is a Type I transmembrane glycoprotein in the immunoglobulin superfamily. Commonly known as Poliovirus Receptor (PVR) due to its involvement in the cellular poliovirus infection in primates, CD155's normal cellular function is in the establishment of intercellular adherens junctions between epithelial cells. The role of CD155 in the immune system is unclear, though it may be involved in intestinal humoral immune responses. Subsequent data has also suggested that CD155 may also be used to positively select MHC-independent T cells in the thymus.

Source
Recombinant Human CD155, Mouse IgG2a Fc Tag, low endotoxin (CD5-H5254) is expressed from human 293 cells (HEK293). It contains AA Trp 21 - Asn 343 (Accession # NP_006496.4).
Predicted N-terminus: Trp 21

Molecular Characterization
This protein carries a mouse IgG2a Fc tag at the C-terminus.
The protein has a calculated MW of 62.2 kDa. As a result of glycosylation, The protein migrates as 70-100 kDa under reducing (R) condition and 130-160 kDa under non-reducing (NR) condition on SDS-PAGE gel.

Endotoxin
Less than 0.1 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
 
Bioactivity
Please refer to product data sheet.

References

(1) Zhang P., et al., 2008, Proc. Natl. Acad. Sci., 105 (47): 18284-18289.
(2) Maier MK., et al., 2007, European Journal of Immunology, 37 (8): 2214-2225.
(3) He Y., et al., 2000, Proc. Natl. Acad. Sci., 97 (1): 79-84.