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Recombinant Mouse BAFFR / TNFRSF13C Protein, Fc Tag, 100µg  

Recombinant Mouse BAFFR / TNFRSF13C Protein, Fc Tag, 100µg

Recombinant Mouse BAFFR / TNFRSF13C, Ser 10 - Ala 71, expressed from human 293 cells (HEK293), Fc Tag (HPLC verified)

Synonym
recombinant, mouse, protein, BAFFR,TNFRSF13C,BAFF-R,BROMIX,CD268,CVID4,prolixin

More details

BAR-M5259-100

Availability: within 7 days

416,00 €

Background
BAFF receptor (B-cell activating factor receptor, BAFF-R), also known as tumor necrosis factor receptor superfamily member 13C (TNFRSF13C), is a membrane protein of the TNF receptor superfamily which recognizes BAFF. B-cell activating factor (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. Overexpression of BAFF in mice results in mature B-cell hyperplasia and symptoms of systemic lupus erythematosus (SLE). Also, some SLE patients have increased levels of BAFF in serum. Therefore, it has been proposed that abnormally high levels of BAFF may contribute to the pathogenesis of autoimmune diseases by enhancing the survival of autoreactive B cells.

Source
Recombinant Mouse BAFFR / TNFRSF13C, Fc Tag (BAR-M5259) is expressed from human 293 cells (HEK293). It contains AA Ser 10 - Ala 71 (Accession # NP_082351.1).
Predicted N-terminus: Ser 10

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 33.3 kDa. As a result of glycosylation, the protein migrates as 40-45 kDa under reducing (R) condition, and 80-100 kDa under non-reducing (NR) condition (SDS-PAGE).

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-HPLC.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
 
Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "BAFFR activates PI3K/AKT signaling in human naive but not in switched memory B cells through direct interactions with B cell antigen receptors"
Sevdali, Block, Lataretu et al
Cell Rep (2022) 39 (13), 111019
(2) "Decreased BAFF Receptor Expression and Unaltered B Cell Receptor Signaling in Circulating B Cells from Primary Sjögren's Syndrome Patients at Diagnosis"
Neys, Verstappen, Bootsma et al
Int J Mol Sci (2022) 23 (9)
(3) "The Alteration of Circulating Lymphocyte Subsets During Tacrolimus Therapy in Neuromyelitis Optica Spectrum Disorder and Its Correlation With Clinical Outcomes"
Wang, Huang, ZhangBao et al
Front Neurol (2021) 12, 816721
Showing 1-3 of 136 papers.