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Recombinant human TIGIT Protein, Fc-Tag, 1mg  

Recombinant human TIGIT Protein, Fc-Tag, 1mg

Recombinant human TIGIT Protein Met 22 - Pro 141 was produced in human 293 cells (HEK293), Fc Tag

Synonym: recombinant, human, protein, TIGIT, VSIG9, VSTM3

More details

TIT-H5254-1K

Availability: within 7 days

2 310,00 €

Background
T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is also known as V-set and immunoglobulin domain-containing protein 9 (VSIG9), V-set and transmembrane domain-containing protein 3 (VSTM3),which belongs to single-pass type I membrane protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level). TIGIT binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.

Source
Recombinant Human TIGIT, Fc Tag (TIT-H5254) is expressed from human 293 cells (HEK293). It contains AA Met 22 - Pro 141 (Accession # Q495A1 -1).
Predicted N-terminus: Met 22

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 39.7 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the Human TIGIT, His Tag by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Bioactivity
Please refer to product data sheet.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

References

(1) Yu X., et al., 2009, Nat. Immunol. 10:48-57.
(2) Stengel K.F., et al., 2012, Proc. Natl. Acad. Sci. U.S.A. 109:5399-5404.
(3) Lozano E., et al., 2012, J. Immunol. 188:3869-3875.