Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant human focal adhesion kinase (FAK), protein kinase, 10 µg  

Recombinant human focal adhesion kinase (FAK), protein kinase, 10 µg

Human FAK (focal adhesion kinase) PTK2 protein tyrosine kinase 2 (PTK2), recombinant and active enzyme

Alternate names: PTK2, FADK, FAK1, pp125FAK, p125FAK, FAK kinase

More details

PK-FAK-A010

Availability: on stock

325,00 €

Background: PTK2 is a focal adhesion-associated protein kinase (FAK) involved in cellular adhesion and spreading processes. FAK, also known as protein tyrosine kinase 2 (PTK2), is a large non-receptor tyrosine kinase that is typically activated through integrin-dependent focal adhesions and controls the assembly and disassembly of focal contacts and cytoskeleton reorganization. FAK is highly enriched in the growth cones of neurons and has a major role in neuronal development. FAK kinase activation leads to the phosphorylation of α-actinin and N-WASP, an actin nucleation-promoting factor that is known to regulate the number of filopodia at the growth cone.

Protein: Recombinant human FAK protein kinase, amino acids A2-H1052, N-terminally fused to GST-Thrombin cleavage site

Theoretical MW : 145.3 kDa (fusion proteins)
Expression system: Baculovirus infected Sf9 cells
Purification: One-step affinity purification using glutathione agarose
Storage buffer: 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol
Protein concentration: 0.135 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MAFC membrane
Specific activity : 1.500 pmol/mg min

Entrez Gene ID: 5747
UniProtKB: Q05397

Ordering information: shipped on dry ice

Product specific literature:

Navarro AI, Rico B. “Focal adhesion kinase function in neuronal development.” Curr Opin Neurobiol. 2014 Apr 2;27C:89-95.

Peer - Reviewed Citations

Heinhuis B1, Koenders MI, van den Berg WB, Netea MG, Dinarello CA, Joosten LA. (2012) "Interleukin 32 (IL-32) contains a typical α-helix bundle structure that resembles focal adhesion targeting region of focal adhesion kinase-1." J Biol Chem. 287(8):5733-43.