ActiveMax® Recombinant Human TGF beta1 Protein, Tag Free, 2x 500 µg

Background: Transforming growth factor beta 1 ( TGFB1) is also known as TGF-β1, CED, DPD1, TGFB. is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation and apoptosis.[1] The TGFB1 protein helps control the growth and division (proliferation) of cells, the process by which cells mature to carry out specific functions (differentiation), cell movement (motility), and the self-destruction of cells (apoptosis). The TGFB1 protein is found throughout the body and plays a role in development before birth, the formation of blood vessels, the regulation of muscle tissue and body fat development, wound healing, and immune system function. TGFB1 is particularly abundant in tissues that make up the skeleton, where it helps regulate bone growth, and in the intricate lattice that forms in the spaces between cells (the extracellular matrix). Within cells, this protein is turned off (inactive) until it receives a chemical signal to become active.[2-3] TGFB1 plays an important role in controlling the immune system, and shows different activities on different types of cell, or cells at different developmental stages. Most immune cells (or leukocytes) secrete TGFB1. [4] TGFB1 has been shown to interact with TGF beta receptor 1, LTBP1, YWHAE, EIF3I and Decorin.

Source
ActiveMax® Human TGFB1, Tag Free (TG1-H4212) is expressed from human 293 cells (HEK293). It contains AA Ala 279 - Ser 390 (Accession # P01137-1). Predicted N-terminus: Ala 279

Molecular Characterization
This protein carries no "tag". The protein has a calculated MW of 12.8 kDa (monomer). the protein migrates as 14 kDa (monomer) under reducing (R) condition, and 25 kDa (Dimer) under non-reducing (NR) condition (SDS-PAGE).

Endotoxin
Less than 1.0 EU per μg of the rhTGFB1 by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM glycine, 50mM NaCl, pH4.0. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
n/a

References

(1) Ghadami M, et al. (2000). Am. J. Hum. Genet. 66 (1): 143–7.
(2) Assoian R, et al. (1983). J Biol Chem , 258 (11): 7155–60.
(3) Derynck R, et al. (1985). Nature 316 (6030): 701–5.
(4) Letterio J, Roberts A (1998). Annu Rev Immunol 16: 137– 61.