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Recombinant Human PDGF-AA Protein, 50µg  

Recombinant Human PDGF-AA Protein, 50µg

Recombinant human Platelet-derived growth factor AA, Ser 87 – Thr211, expressed in human 293 cells (HEK293)

Synonyms: recombinant, human, protein, PDGFAA, PDGF-AA, PDGF1, PDGF-1

More details

PDA-H4218-050

Availability: within 7 days

420,00 €

Background: PDGFs are mitogenic during early developmental stages, driving the proliferation of undifferentiated mesenchyme and some progenitor populations. During later maturation stages, PDGF signalling has been implicated in tissue remodelling and cellular differentiation, and in inductive events involved in patterning and morphogenesis. In addition to driving mesenchymal proliferation, PDGFs have been shown to direct the migration, differentiation and function of a variety of specialised mesenchymal and migratory cell types, both during development and in the adult animal. Other growth factors in this family include vascular endothelial growth factors B and C (VEGF-B, VEGF-C)which are active in angiogenesis and endothelial cell growth, and placenta growth factor (PlGF) which is also active in angiogenesis.(1-3) PDGF plays a role in embryonic development, cell proliferation, cell migration, and angiogenesis. PDGF is a required element in cellular division for fibroblast, a type of connective tissue cell. PDGF is also known to maintain proliferation of oligodendrocyte progenitor cells.(4) Platelet-derived growth factor subunit A is also known as PDGFA, PDGF-A, PDGF1, is a member of the platelet-derived growth factor family. PDGFA can exist either as a homodimer (PDGF-AA) or as a heterodimer with the platelet-derived growth factor Bata polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds.

Source
Recombinant human Platelet-derived growth factor AA ( rhPDGF-AA) Ser 87 – Thr211(Accession # P04085) was expressed in human 293 cells (HEK293)

Molecular Characterization
rhPDGF-AA is a homodimeric, glycosilated, polypeptide chain containing 2×110 amino acids, and has a calculated MW of 29 kDa. In DTT-reduced SDS-PAGE, rhPDGF-AA protein migrates as 16.5 kDa poly peptide due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhPDGF-AA by the LAL method.

Purity
>95% purity as determined by SDS-PAGE of reduced rhPDGF-AA.

Formulation
Lyophilized from 0.22 μm filtered solution in 50mM Tris 150mM NaCl pH 7.0. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse 3T3 cells.The ED50was 4-10 ng/ml.

References

(1) Hoch RV, Soriano P. 2003, Development 130 (20): 4769– 4784
(2) Joukov V, et al. 1996, Proc. Natl. Acad. Sci. U.S.A. 93 (6): 2567–2581
(3) Joukov V, et al. 1996, EMBO J. 15 (2): 290–298
(4) Lei KJ, et al. 1993, Oncogene 8 (4): 925–931