Products

Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant Human VEGF121 Protein, His Tag, 1mg  

Recombinant Human VEGF121 Protein, His Tag, 1mg

Recombinant Human VEGF121 Protein, Ala 27 - Arg 147, expressed in human 293 cells (HEK293)

Synonym: recombinant human protein, VEGF121,VEGFA,VPF

More details

VE1-H5246-1K

Availability: within 7 days

2 500,00 €

Background
Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) and VEGF-A, and is a member of the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. Alternatively spliced isoforms of 121,145,165,183,189 and 206 amino acids in length are expressed in humans. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189. VEGF121 is the only form that lacks a basic heparinbinding region and is freely diffusible. Mouse embryos expressing only the corresponding isoform (VEGF120) do not survive to term, and show defects in skeletogenesis. Human VEGF121 shares 87% aa sequence identity with corresponding regions of mouse and rat, 93% with feline, equine and bovine, and 91%, 95% and 96% with ovine, canine and porcine VEGF, respectively. VEGF121 induces the proliferation of lymphatic endothelial cells. The lymphangiogenesis may be promoted by upregulation of VEGF121, which may in turn act in part via induction of VEGF-C.

Source
Recombinant Human VEGF121, His Tag (VE1-H5246) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 147 (Accession # P15692-9).
Predicted N-terminus: His

Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 15.0 kDa. As a result of glycosylation, the protein migrates as 17 kDa and 20 kDa under reducing (R) condition, and 35-45 kDa under non-reducing (NR) condition (SDS-PAGE).

Endotoxin
Less than 1.0 EU per µg of the Human VEGF121, His Tag by the LAL method.

Purity
>95% as determined by reduced SDS-PAGE.

Formulation
Lyophilized from 0.22 µm filtered solution in PBS, pH7.4. Normally Trehalose are added as protectants before lyophilization.
Contact us for customized product form or formulation.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

Bioactivity
Immobilized Human VEGFR1/R2, Fc Tag at 1 μg/mL (100 μL/well) can bind Human VEGF121, His Tag (Cat. No. VE1-H5246) with a linear range of 2-40 ng/mL (QC tested).

References

(1) Byrne, A.M. et al., 2005, J. Cell. Mol. Med, 9(4):777-94.
(2) Robinson, C.J. et al., 2001, J. Cell. Sci. 114(Pt 5):853-65.
(3) Zelzer, E. et al., 2002, Development, 129(8):1893-904.

The following products could also be interesting for you: