kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
ActiveMax® Recombinant Human IL-6 protein, 20µg  

ActiveMax® Recombinant Human IL-6 protein, 20µg

ActiveMax® Recombinant Human IL-6, Val 30 - Met 212, produced in human 293 cells (HEK293), Tag free

Synonym: Recombinant Human Protein, IL6,HGF,Interleukin-6,BSF2,HSF,IFNB2

More details


Availability: within 7 days

192,00 €

Interleukin 6 (IL-6) is also known as HGF, BSF2,HSF, IFNB2 and IL-6, originally identified as a B cell differentiation factor, is a multifunctional cytokine that regulates immune responses, hematopoiesis, acute phase responses, and inflammatory reactions.It is secreted by T cells, macrophages , monocytes, fibroblasts,endothelial cells, to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. Interleukin 6 has been shown to interact with interleukin-6 receptor and glycoprotein. IL-6 is relevant to many disease processes such as diabetes,atherosclerosis, depression,Alzheimer's Disease,systemic,lupus erythematosus,prostate cancer and rheumatoid arthritis. Advanced/metastatic cancer patients have higher levels of IL-6 in their blood.Hence there is an interest in developing anti-IL-6 agents as therapy against many of these diseases.

ActiveMax® Recombinant Human IL-6 Protein, Val 30 - Met 212 (Accession # NP_000591) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization
ActiveMax® Human IL-6 contains no "tag", and has a calculated MW of 20.8 kDa. The predicted N-terminus is Val 30 . The reducing (R) protein migrates as 24-27 kDa in SDS-PAGE due to different glycosylation.

Less than 1.0 EU per μg by the LAL method.

>98% as determined by SDS-PAGE.

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).
Measured by its binding ability in a functional ELISA. Immobilized Human IL-6, Tag Free (Catalog # IL6-H4218) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-6 R alpha, His Tag (Catalog # CD6-H82E8) with a linear range of 4-125 ng/mL.


(1) Ferguson-Smith AC, 1988, Genomics, 2 (3): 203–208.
(2) Schwantner A,et. al. 2004, J. Biol. Chem. 279 (1): 571–6.
(3) Schuster B,et. al. 2003, J. Biol. Chem. 278 (11): 9528–35.
(4) Taga T,et al.1989, Cell 58 (3): 573–81.