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ActiveMax® Recombinant Human IL-6 protein, 20µg  

ActiveMax® Recombinant Human IL-6 protein, 20µg

ActiveMax® Recombinant Human IL-6, Val 30 - Met 212, produced in human 293 cells (HEK293), Tag free

Synonym: Recombinant Human Protein, IL6,HGF,Interleukin-6,BSF2,HSF,IFNB2

More details

IL6-H4218-20

Availability: within 7 days

192,00 €

Background
Interleukin 6 (IL-6) is also known as HGF, BSF2,HSF, IFNB2 and IL-6, originally identified as a B cell differentiation factor, is a multifunctional cytokine that regulates immune responses, hematopoiesis, acute phase responses, and inflammatory reactions.It is secreted by T cells, macrophages , monocytes, fibroblasts,endothelial cells,et.al. to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. Interleukin 6 has been shown to interact with interleukin-6 receptor and glycoprotein. IL-6 is relevant to many disease processes such as diabetes,atherosclerosis, depression,Alzheimer's Disease,systemic,lupus erythematosus,prostate cancer and rheumatoid arthritis. Advanced/metastatic cancer patients have higher levels of IL-6 in their blood.Hence there is an interest in developing anti-IL-6 agents as therapy against many of these diseases.

Source
ActiveMax® Recombinant Human IL-6 Protein, Val 30 - Met 212 (Accession # NP_000591) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization
ActiveMax® Human IL-6 contains no "tag", and has a calculated MW of 20.8 kDa. The predicted N-terminus is Val 30 . The reducing (R) protein migrates as 24-27 kDa in SDS-PAGE due to different glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>98% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).
 Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized Human IL-6, Tag Free (Catalog # IL6-H4218) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-6 R alpha, His Tag (Catalog # CD6-H82E8) with a linear range of 4-125 ng/mL.

References

(1) Ferguson-Smith AC, et.al. 1988, Genomics, 2 (3): 203–208.
(2) Schwantner A,et. al. 2004, J. Biol. Chem. 279 (1): 571–6.
(3) Schuster B,et. al. 2003, J. Biol. Chem. 278 (11): 9528–35.
(4) Taga T,et al.1989, Cell 58 (3): 573–81.