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ActiveMax® Recombinant Human SCF /KITLG protein, 20µg  

ActiveMax® Recombinant Human SCF /KITLG protein, 20µg

ActiveMax® Recombinant Human SCF /KITLG Protein, Glu 26 - His 214, produced in human 293 cells (HEK293), Tag free

Synonym: Recombinant Human Protein, KITLG,FPH2,KL-1,Kitl,MGF,SCF,SF,SHEP7,KL

More details

SCF-H4212-20

Availability: within 7 days

237,00 €

Background
Stem Cell Factor is also known as SCF, kit-ligand, KL, steel factor, KITLG, FPH2, KL-1, Kitl, MGF, SCF, SF, or SHEP7, and is a cytokine that binds to the c-Kit receptor (CD117). SCF can exist both as a transmembrane protein and a soluble protein. This cytokine plays an important role in hematopoiesis (formation of blood cells), spermatogenesis, and melanogenesis. The soluble and transmembrane forms of the protein are formed by alternative splicing of the same RNA transcript.Soluble and transmembrane SCF is produced by fibroblasts and endothelial cells. Soluble SCF has a molecular weight of 18,5 KDa and forms a dimer. SCF plays an important role in the hematopoiesis during embryonic development. Sites where hematopoiesis takes place, such as the fetal liver and bone marrow, all express SCF. During development, the presence of the SCF also plays an important role in the localization of melanocytes, cells that produce melanin and control pigmentation. SCF plays a role in the regulation of HSCs in the stem cell niche in the bone marrow. SCF may be used along with other cytokines to culture HSCs and hematopoietic progenitors. The expansion of these cells ex-vivo (outside the body) would allow advances in bone-marrow transplantation, in which HSCs are transferred to a patient to re-establish blood formation.

Source
ActiveMax® Recombinant Human SCF, Tag Free (SCF-H4212) is expressed from human 293 cells (HEK293). It contains AA Glu 26 - His 214 (Accession # AAH69797).
Predicted N-terminus: Glu 26

Molecular Characterization
This protein carries no "tag".
The protein has a calculated MW of 21.0 kDa. The protein migrates as 32-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>98% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). 

Bioactivity
Please refer to product data sheet.

References

(1) Anderson DM, et al., 1991, Cell Growth Differ. 2 (8): 373–8.
(2) Broudy VC, 1997, Blood 90 (4): 1345–64.
(3) Blouin R, Bernstein A, 1993, In Freedman MH, Feig SA. Boca Raton: CRC Press.
(4) Kent D, et al., 2008, Clin. Cancer Res. 14 (7): 1926–30.