Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
ActiveMax® Recombinant Human IL-2 Protein, 2 x 500 µg  

ActiveMax® Recombinant Human IL-2 Protein, 2 x 500 µg

ActiveMax® Recombinant Human IL-2 Protein, Ala 21 - Thr 153, produced in human 293 cells (HEK293), Tag free

Synonym: Recombinant Human Protein, IL2, lymphokine,Interleukin 2

More details

IL2-H4216-1K

Availability: within 7 days

1 078,00 €

Background
Interleukin-2 (IL-2) is an interleukin, a type of cytokine immune system signaling molecule, which is a leukocytotrophic hormone that is instrumental in the body's natural response to microbial infection and in discriminating between foreign (non-self) and self. IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes, the cells that are responsible for immunity. Mature human IL-2 shares 56% and 66% aa sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit crossspecies activity. The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes. IL-2 is also necessary during T cell development in the thymus for the maturation of a unique subset of T cells that are termed regulatory T cells (T-regs). After exiting from the thymus, T-Regs function to prevent other T cells from recognizing and reacting against "self antigens", which could result in "autoimmunity". T-Regs do so by preventing the responding cells from producing IL-2. Thus, IL-2 is required to discriminate between self and non-self, another one of the unique characteristics of the immune system.

Source
ActiveMax® Recombinant Human IL-2 Protein, Ala 21 - Thr 153 (Accession # P60568) was produced in human 293 cells (HEK293)

Molecular Characterization
This protein carries no "tag".
The protein has a calculated MW of 15.4 kDa. The protein migrates as 17 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH5.0. Normally Trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). 

Bioactivity
Immobilized Human IL-2, Tag Free (Cat. No. IL2-H4216) at 10μg/mL (100 μL/well) can bind Human IL-2 R beta, Fc Tag (Cat. No. ILB-H5253) with a linear range of 0.625-5 μg/mL (Routinely tested).
Request for protocol
Immobilized Human IL-2 R alpha, Fc Tag (Cat. No. ILA-H5251) at 2μg/mL (100 μL/well) can bind Human IL-2, Tag Free (Cat. No. IL2-H4216) with a linear range of 0.2-1.56 ng/mL (QC tested).
Request for protocol

References

(1) Gaffen, S.L. and K.D. Liu, 2004, Cytokine 28:109.
(2) Kasakura S, Lowenstein L, 1965, Nature 208 (5012): 794–795.
(3) Smith KA, Favata MF, Oroszlan S, 1983, J. Immunol. 131 (4): 1808.
(4) Thornton AM, Shevach EM, 1998, J. Exp. Med. 188 (2): 287–96.

The following products could also be interesting for you: