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ActiveMax® Recombinant Human LIF Protein, 1 mg  

ActiveMax® Recombinant Human LIF Protein, 1 mg

ActiveMax® Recombinant Human Leukemia inhibitory factor / LIF protein Ser 23 - Phe 202, produced in human 293 cells (HEK293)

Synonym: Recombinant Human Protein, LIF

More details

LIF-H521b-1K

Availability: within 7 days

2 250,00 €

Background
Leukemia inhibitory factor, or LIF, an interleukin 6 class cytokine, is a protein in cells that affects cell growth and development.Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. Therefore LIF is used in mouse embryonic stem cell culture. It is necessary to maintain the stem cells in an undifferentiated state, however genetic manipulation of embryonic stem cells allows for LIF independent growth, notably overexpression of the gene Nanog. LIF is not required for culture of human embryonic stem cells.

Source
ActiveMax® Recombinant Human LIF Protein (ActiveMax® Human LIF) Ser 23 - Phe 202 (Accession # AAH69540.1) was produced in human 293 cells (HEK293)

Molecular Characterization
rhLIF, with Gly-Pro at the N- terminus, has a calculated MW of 19.9 kDa. The predicted N-terminus is Ser 23. DTT-reduced Protein migrates as 33-45 kDa.

Endotoxin
Less than 1.0 EU per μg of the ActiveMax® Human LIF by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). 

Bioactivity
Determined by its ability to stimulate the proliferation of human TF-1 cells.
The ED50 <0.1 ng/mL, corresponding to a specific activity of >1X107 Unit/mg.

References

(1) Hu W,et al., 2007, Nature, 450 (7170): 721-4.
(2) Kawahara Y,et al., 2009, PLoS One, 4 (7): e6343.
(3) Patterson PH (1994). Proc. Natl. Acad. Sci. U.S.A. 91 (17).