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ActiveMax® Recombinant Human GFER /ALR, 20 µg  

ActiveMax® Recombinant Human GFER /ALR, 20 µg

Recombinant Human GFER /HPO Protein, ActiveMax, produced in human 293 cells (HEK293), Tag free

Synonym: Recombinant Human Protein, GFER,ALR,ERV1,HERV1,HPO,Hepatopoietin

More details

GFR-H521b-20

Availability: within 7 days

300,00 €

Background
Growth factor, augmenter of liver regeneration (GFER) is also known as FAD-linked sulfhydryl oxidase ALR, which belongs to the Erv1/ALR family of proteins. This family can be found in higher and lower eukaryotes. There are two isoform of GFER: Isoform 1 and isoform 2. Isoform 2 missing 1 – 80 aa. Isoform 1 is mainly located in mitochondrion intermembrane space, while Isoform 1 is secreted to cytoplasm. Isoform 1 of GFER regenerates the redox-active disulfide bonds in CHCHD4/MIA40, a chaperone essential for disulfide bond formation and protein folding in the mitochondrial intermembrane space. The reduced form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with GFER/ERV1, resulting in regeneration of the essential disulfide bonds in CHCHD4/MIA40, while GFER/ERV1 becomes re-oxidized by donating electrons to cytochrome c or molecular oxygen. The isoform 2 of GFER may act as an autocrine hepatotrophic growth factor promoting liver regeneration.

Source
Recombinant Human GFER /HPO Protein,HEK293 expressed,ActiveMax (rh GFER) Met 81 - Asp 205 (Accession # NP_005253) was produced in human 293 cells (HEK293)

Molecular Characterization
rh GFER contains no "tag", and has a calculated MW of 15.2 kDa. The predicted N-terminus is Met 81. DTT-reduced Protein migrates as 16 kDa in SDS-PAGE .

Endotoxin
Less than 1.0 EU per μg of the rh GFER by the LAL method.

Purity
>92% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

References

(1) Daithankar V.N., et al., 2009, Biochemistry 48:4828-4837.
(2) Sztolsztener M.E., et al., 2013, Traffic 14:309-320.
(3) Di Fonzo A., et al., 2009, Am. J. Hum. Genet. 84:594-604.