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Recombinant Human IL-23 alpha & IL-12 beta Heterodimer Protein, 2x500 µg  

Recombinant Human IL-23 alpha & IL-12 beta Heterodimer Protein, 2x500 µg

Recombinant Human IL-23 alpha & IL-12 beta Heterodimer Protein, Arg 20 - Pro 189 (IL23A) & Ile 23 - Ser 328 (IL12B), produced in human 293 cells (HEK293), His Tag&Tag Free

Synonym: Recombinant huma protein,IL-23 alpha & IL-12 beta

More details

ILB-H52W5-1K

Availability: within 7 days

3 500,00 €

Background
Interleukin-23 subunit alpha (IL-23 alpha) can associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.

Source
Recombinant Human IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free (ILB-H52W5) is expressed from human 293 cells (HEK293). It contains AA Arg 20 - Pro 189 (IL23A) & Ile 23 - Ser 328 (IL12B) (Accession # Q9NPF7-1 (IL23A) & P29460-1 (IL12B)).
Predicted N-terminus: His (IL23A) & Ile 23 (IL12B)

Molecular Characterization
Human IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free, produced by co-expression of IL-23 alpha and IL-12 beta, has a calculated MW of 19.5 kDa (IL-23 alpha) and 34.7 kDa (IL-12 beta). Subunit IL-23 alpha is fused with a polyhistidine tag at the N-terminus and subunit IL-12 beta contains no tag. The reducing (R) protein migrates as 20 kDa (IL-23 alpha) and 37-45 kDa (IL-12 beta) respectively due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

References

(1) Oppmann B., et al., 2000, Immunity 13:715-725.
(2) Parham C., et al., 2002, J. Immunol. 168:5699-5708.