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Background
Chorionic gonadotrophin subunit alpha (CGA),a member of the glycoprotein hormones subunit alpha family, is also known as glycoprotein hormones alpha chain, choriogonadotropin alpha chain, follicle-stimulating hormone alpha chain (FSH-alpha), luteinizing hormone alpha chain (LSH-alpha) and thyroid-stimulating hormone alpha chain (TSH-alpha). The hormones human chorionic gonadotropin (hCG), luteinizing hormone (LH), follicle-stimulating hormone (FSH), and thyroid-stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits (CGA) of these four human glycoprotein hormones are nearly identical, however, their beta chains are unique and confer biological specificity.
Source
Recombinant Human CGA /TSH alpha Protein, His Tag (CGA-H52H2) is expressed from human 293 cells (HEK293). It contains AA Ala 25 - Ser 116 (Accession # AAH20782).
Predicted N-terminus: Ala 25
Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 12.1 kDa. The protein migrates as 22-26 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
(1) "Comparative outcomes of extracorporeal membrane oxygenation for COVID-19 delivered in experienced European centres during successive SARS-CoV-2 variant outbreaks (ECMO-SURGES): an international, multicentre, retrospective cohort study"
Schmidt, Hajage, Landoll et al
Lancet Respir Med (2023)
(2) "Effect of autologous platelet-rich plasma combined with sodium hyaluronate on clinical efficacy and serum inflammatory factors in patients with knee osteoarthritis"
Zhou, Feng, Lei et al
Am J Transl Res (2022) 14 (12), 8724-8732
(3) "Therapeutic effect of captopril combined with phosphocreatine sodium on viral myocarditis"
Wang, Chen, Li et al
Am J Transl Res (2022) 14 (12), 8659-8667
Showing 1-3 of 2795 papers.
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