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Recombinant Human IL-17E / IL-25 Protein, His Tag, 20µg  

Recombinant Human IL-17E / IL-25 Protein, His Tag, 20µg

Recombinant Human IL-17E / IL-25 Protein, Tyr 33 - Gly 177, produced in human 293 cells (HEK293), His Tag

Synonyms: Recombinant human protein, IL-17E,IL-25,Interleukin-25

More details

IL5-H4221-20

Availability: within 7 days

192,00 €

Background
Interleukin 25 (IL25) is also known as Interleukin-17E (IL-17E), is a distinct member of the IL17 cytokine family comprised of at least six members sharing a conserved cysteine-knot structure but divergent at the N-terminus. IL-25 is secreted by type 2 helper T cells (Th2) and mast cells. IL25 can induce NF-κB activation, and stimulate the production of IL8. Both this cytokine and IL17B are ligands for the cytokine receptor IL17RB. IL-25 induces the production of other cytokines, including IL-4, IL-5 and IL-13 in multiple tissues, which stimulate the expansion of eosinophils. This cytokine is an important molecule controlling immunity of the gut and has been implicated in chronic inflammation associated with the gastrointestinal tract. IL-25 can kill some types of breast cancer cells.

Source
Recombinant Human IL-17E / IL-25 Protein (Human IL-17E, His Tag) Tyr 33 - Gly 177 (Accession # NP_073626.1) was produced in human 293 cells (HEK293)

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 16.7 kDa. The protein migrates as 23-28 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation..

Endotoxin
Less than 1.0 EU per μg of the Human IL-17E, His Tag by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).
 
Bioactivity
The activity is determined by the dose-dependant production of IL-8 by human PBMCs.
The expected ED50 for this effect is 12-25 ng/mL.

References

(1) Ikeda K., et al., 2003, Blood 101 (9): 3594–6.
(2) Owyang AM., et al., 2006, J. Exp. Med. 203 (4): 843–9.
(3) Furuta S., et al., 2011, Sci Transl Med 3 (78): 78ra31.