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Recombinant Human Latent TGF-beta 1 / TGFB1 Protein, His tag, 2 x 500µg  

Recombinant Human Latent TGF-beta 1 / TGFB1 Protein, His tag, 2 x 500µg

Recombinant Human Latent TGFB1 (Leu 30 - Ser 390), expressed from human 293 cells (HEK293), predicted N-terminus: His & Ala 279, His tag (MALS verified)

Synonym
recombinant, human, Latent TGF-beta 1,Latent TGFB1,TGFB1,CED,DPD1,LAP,TGF-beta-1,TGFB

More details

TG1-H524x-1K

Availability: within 7 days

2 980,00 €

Background
Transforming growth factor beta 1 ( TGFB1) is also known as TGF-β1, CED, DPD1, TGFB. is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation and apoptosis. The TGFB1 protein helps control the growth and division (proliferation) of cells, the process by which cells mature to carry out specific functions (differentiation), cell movement (motility), and the self-destruction of cells (apoptosis). The TGFB1 protein is found throughout the body and plays a role in development before birth, the formation of blood vessels, the regulation of muscle tissue and body fat development, wound healing, and immune system function. TGFB1 is particularly abundant in tissues that make up the skeleton, where it helps regulate bone growth, and in the intricate lattice that forms in the spaces between cells (the extracellular matrix). Within cells, this protein is turned off (inactive) until it receives a chemical signal to become active. TGFB1 plays an important role in controlling the immune system, and shows different activities on different types of cell, or cells at different developmental stages. Most immune cells (or leukocytes) secrete TGFB1. TGFB1 has been shown to interact with TGF beta receptor 1, LTBP1, YWHAE, EIF3I and Decorin.

Source
Recombinant Human Latent TGFB1, His Tag (TG1-H524x) is expressed from human 293 cells (HEK293). It contains AA Leu 30 - Ser 390 (Accession # P01137-1).
Predicted N-terminus: His

Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 42.6 kDa (monomer). The protein migrates as 37-45 kDa (LAP) and 13 kDa (TGFB1) under reducing (R) condition (SDS-PAGE) due to glycosylation and Interchain disulfide bond.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

References

(1) TGF-β1 facilitates MT1-MMP-mediated proMMP-9 activation and invasion in oral squamous cell carcinoma cells
Yamahana, Terashima, Takatsuka et al
Biochem Biophys Rep (2021) 27, 101072
(2) Photoactivation of TGFβ/SMAD signaling pathway ameliorates adult hippocampal neurogenesis in Alzheimer's disease model
Wu, Shen, Zhang et al
Stem Cell Res Ther (2021) 12 (1), 345
(3) Identification of Potential Pathogenic Super-Enhancers-Driven Genes in Pulmonary Fibrosis
Li, Zhao, Li et al
Front Genet (2021) 12, 644143
Showing 1-3 of 1019 papers.
Please refer to product data sheet.