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Recombinant Human AIMP1 / EMAP2 / SCYE1 Protein, 1mg  

Recombinant Human AIMP1 / EMAP2 / SCYE1 Protein, 1mg

Recombinant Human AIMP1 / EMAP2 / SCYE1 Protein (Ala 2 - Lys 312), produced in E.coli cells, His tag

Synonym
recombinant, human, AIMP1, EMAP2, EMAPII, HLD3, SCYE1, p43, EMAP-2, EMAP-II, AIMP-1

More details

EM2-H5142-1K

Availability: within 7 days

1 800,00 €

Background
Aminoacyl tRNA synthase complex-interacting multifunctional protein 1 (AIMP1) is also known as multisynthase complex auxiliary component p43 and endothelial monocyte-activating polypeptide II (EMAP-II). AIMP1 is a cytokine that may be induced by apoptosis and is also released from professional antigen-presenting cells such as dendritic cells. The release of AIMP1 renders the tumor-associated vasculature sensitive to tumor necrosis factor. Furthermore, AIMP1 binds tRNA and stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase. This protein possesses inflammatory cytokine activity. Also, AIMP1 negatively regulates TGF-beta signaling through stabilization of SMURF2 by binding to SMURF2 and inhibiting its SMAD7-mediated degradation.

Source
Recombinant Human AIMP1, His Tag (EM2-H5142) is expressed from E.coli cells. It contains AA Ala 2 - Lys 312 (Accession # Q12904-1).
Predicted N-terminus: Met

Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 35.2 kDa. The protein migrates as 36 kDa under reducing (R) condition (SDS-PAGE).

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

References

(1) Barnett G., et al., 2000, Cancer Res. 60:2850-2857.
(2) Park S.G., et al., 1999, J. Biol. Chem. 274:16673-16676.
(3) Tandle A.T., et al., 2009, Exp. Cell Res. 315:1850-1859.