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Recombinant Human Gremlin / GREM1 Protein, Fc Tag, 1mg  

Recombinant Human Gremlin / GREM1 Protein, Fc Tag, 1mg

Recombinant Human Gremlin / GREM1 (Lys 25 - Asp 184), expressed from human 293 cells (HEK293), Fc tag

Synonym
recombinant, human, protein, GREM1,CKTSF1B1,DAND2,DRM,PIG2

More details

GR1-H5254-1K

Availability: within 7 days

2 100,00 €

Background
Gremlin is also known as Cysteine knot superfamily 1, BMP antagonist 1 (CKTSF1B1), DAN domain family member 2 (DAND2), Down-regulated in Mos-transformed cells protein (DRM), Increased in high glucose protein 2 (IHG-2), Cell proliferation-inducing gene 2 protein (PIG2) or Gremlin-1 (GREM1), which is highly expressed in small intestine, fetal brain and colon. Gremlin / GREM-1 interacts with SLIT1 and SLIT2 in a glycosylation-dependent manner. Gremlin may play an important role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. Gremlin down-regulates the BMP4 signaling in a dose-dependent manner and acts as inhibitor of monocyte chemotaxis.

Source
Recombinant Human Gremlin / GREM1, Fc Tag (GR1-H5254) is expressed from human 293 cells (HEK293). It contains AA Lys 25 - Asp 184 (Accession # NP_037504).
Predicted N-terminus: Lys 25

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 44.2 kDa. The protein migrates as 48-60 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 150 mM NaCl, pH7.5 with 1 mM EDTA. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
 
Bioactivity (ELISA bioactivity)
Immobilized Human BMP-2, Tag Free (Catalog # BM2-H4117) at 2μg/mL (100 μL/well) can bind Human Gremlin, Fc Tag (Catalog # GR1-H5254) with a linear range of 0.0008-0.08 μg/mL.

References

(1) McMahon R., et al., 2000, J. Biol. Chem. 275:9901-9904.
(2) Topol L.Z., et al., 2000, Cytogenet. Cell Genet. 89:79-84.
(3) Jaeger E., et al., 2012, Nat. Genet. 44:699-703.