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ActiveMax® Recombinant Human TNF-alpha (HPLC-verified), 2 x 500µg  

ActiveMax® Recombinant Human TNF-alpha (HPLC-verified), 2 x 500µg

ActiveMax® Recombinant Human TNF-alpha (ActiveMax® Human TNF-alpha) Val 77 - Leu 233 was produced in human 293 cells (HEK293)

Synonyms: TNF, DIF, TNF-alpha, TNFalpha, TNFA, TNFSF2, TNF-A, cachexin, cachectin, TNF-a, TNFa, tumor-necrosis-factor-alpha, TNFa cytokine, TNF alpha protein

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TNA-H4211-1K

Availability: within 7 days

1 050,00 €

Background: Tumor necrosis factor alpha (TNFα) is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimer's disease and cancer. Recombinant TNFα is used as an immunostimulant under the INN tasonermin. TNFα can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.

Source
ActiveMax® Recombinant Human TNF-alpha (ActiveMax® Human TNF-alpha) Val 77 - Leu 233 (Accession # NP_000585.2) was produced in human 293 cells (HEK293).

Molecular Characterization
ActiveMax® Human TNF-alpha contains no "tag", and has a calculated MW of 17.4 kDa. The predicted N-terminus is Val 77. The reducing (R) protein migrates as 18 kDa in SDS-PAGE .
A SEC-HPLC analysis showing 95% of ActiveMax® Recombinant Human TNF-alpha (TNA-H4211) present as active trimers with a molecular mass of 54 kDa.

Endotoxin
Less than 1.0 EU per μg of the ActiveMax® Human TNF-alpha by the LAL method.

Purity
>95% as determined by SEC-HPLC.
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
Measured by its binding ability in a functional ELISA.
Immobilized ActiveMax® Human TNF-alpha (Catalog # TNA-H4211) at 5 μg/mL ( 100 μl/well ) can bind Humira with a linear range of 0.04-0.6 ng/mL.
SEC-HPLC
A SEC-HPLC analysis showing over 95% of ActiveMax® Recombinant Human TNF-alpha (TNA-H4211) present as active trimers with a molecular mass of 54 kDa.

References

(1) Dowlati Y, Herrmann N, et al., 2010, Biol Psychiatry 67 (5): 446–457.
(2) Swardfager W, Lanctot K, et al., 2010, Biol Psychiatry 68 (10): 930–941.
(3) Locksley RM, Killeen N et al., 2001, Cell 104 (4): 487–501.