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Recombinant Human IL-8 / CXCL8 Protein, His Tag, 1 mg (2x 500 µg)  

Recombinant Human IL-8 / CXCL8 Protein, His Tag, 1 mg (2x 500 µg)

Recombinant Human IL-8 / CXCL8 Protein, expressed from human HEK293 cells, contains AA Ser 28 - Ser 99, His Tag

Synonym
recombinant, human, protein, CXCL8, GCP1, IL8, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1

More details

IL8-H52H3-1K

Availability: within 7 days

2 310,00 €

Background
Interleukin 8 (IL8 or chemokine (C-X-C motif) ligand 8, CXCL8) is a chemokine produced by macrophages and other cell types such as epithelial cells, airway smooth muscle cells and endothelial cells. There are many receptors on the surface membrane capable of binding IL-8, the most frequently studied types are the G protein-coupled serpentine receptors CXCR1 and CXCR2. Expression and affinity for IL-8 differs between the two receptors (CXCR1 > CXCR2). IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.

Source
Recombinant Human IL-8, His Tag (IL8-H52H3) is expressed from human 293 cells (HEK293). It contains AA Ser 28 - Ser 99 (Accession # P10145-1).
Predicted N-terminus: Ser 28

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 10.3 kDa. The protein migrates as 13 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.
 
Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution. 

References

(1)  Hedges JC. 2000. Am J Respir Cell Mol Biol. 23(1):86-94.
(2)  Van Damme J. 1990. Eur J Immunol. 20(9):2113-8.
(3)  Hébert CA. 1990. J Immunol. 145(9):3033-40.