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Recombinant Biotinylated Human IL-17A / CTLA-8 Protein, His, Avitag™, 200 µg  

Recombinant Biotinylated Human IL-17A / CTLA-8 Protein, His, Avitag™, 200 µg

Recombinant Biotinylated Human IL-17A / CTLA-8 Protein, expressed from human HEK293 cells, contains AA Gly 24 - Ala 155, His and Avitag™

Synonym
recombinant, human, protein, IL-17A, Interleukin-17A, CTLA-8, IL-17

More details

ILA-H82Q1-200

Availability: within 7 days

1 380,00 €

Background
Interleukin-17A (IL17A) is also known as cytotoxic T-lymphocyte-associated antigen 8 (CTLA8),which is a proinflammatory cytokine produced by activated T cells. IL17A can regulate the activities of NF-kappaB and mitogen-activated protein kinases. Also,IL17A can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO).Furthermore,IL17A has been found both in glycosylated and nonglycosylated forms. High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.

Source
Recombinant Biotinylated Human IL17A, His,Avitag (ILA-H82Q1) is expressed from human 293 cells (HEK293). It contains AA Gly 24 - Ala 155 (Accession # Q16552-1).
Predicted N-terminus: His

Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 17.8 kDa. The protein migrates as 19-21 kDa and 23-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.
 
Purity
>90% as determined by SDS-PAGE.

Biotinylation
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Biotin:Protein Ratio
The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution. 

Bioactivity-ELISA
Immobilized Human IL-17 RA, Fc Tag (Cat. No. ILA-H5257) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL17A, His,Avitag (Cat. No. ILA-H82Q1) with a linear range of 0.3-10 ng/mL (QC tested).
Immobilized Monoclonal Anti-Human IL-17A Antibody, Human IgG1 at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL17A, His,Avitag (Cat. No.ILA-H82Q1) with a linear range of 0.3-2 ng/mL (Routinely tested).

References

(1)  Yao Z., et al.,1995, J. Immunol. 155:5483-5486.
(2)  Gerhardt S., et al., 2009, J. Mol. Biol. 394:905-921.