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Recombinant Human IL-10 Protein, His Tag, 1 mg (4x250µg)  

Recombinant Human IL-10 Protein, His Tag, 1 mg (4x250µg)

Recombinant Human IL-10 Protein, Ser 19 - Asn 178, produced in human 293 cells (HEK293), His Tag

Synonym: Recombinant human protein, CSIF, IL-10, IL10A, TGIF, B-TCGF, GVHDS, MGC126450, MGC126451, RP11-262N9.1, Interleukin-10 

More details

IL0-H4248-1K

Availability: within 7 days

4 420,00 €

Background
Interleukin-10 (IL-10) is also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. IL-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. Mature human IL-10 shares 72% - 86% amino acid sequence identity with bovine, canine, equine, feline, mouse, ovine, porcine, and rat IL-10. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells. IL-10 is capable of inhibiting synthesis of pro-inflammatory cytokines such as IFN-γ, IL-2, IL-3, TNFα and GM-CSF made by cells such as macrophages and regulatory T-cells. It also displays a potent ability to suppress the antigen-presentation capacity of antigen presenting cells. However, it is also stimulatory towards certain T cells and mast cells and stimulates B cell maturation and antibody production. Knockout studies suggested the function of Interleukin-10 / IL-10 as an essential immunoregulator in the intestinal tract. Patients with Crohn's disease react favorably towards treatment with bacteria producing recombinant interleukin-10, showing the importance of interleukin-10 for counteracting excessive immunity in the human body.

Source
Recombinant Human IL-10 Protein (Human IL-10), His Tag (IL0-H4248) is expressed from human 293 cells (HEK293). It contains AA Ser 19 - Asn 178 (Accession # NP_000563.1).
Predicted N-terminus: His

Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 19.5 kDa. The protein migrates as 19 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the Human IL-10, His Tag by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "IL-10 inhibits osteoclast differentiation and osteolysis through MEG3/IRF8 pathway"
Gao, Ge, Zhou et al
Cell Signal (2022)
(2) "Plasma cytokines during pregnancy provide insight into the risk of diabetes in the gestational diabetes risk group"
Tagoma, Haller-Kikkatalo, Oras et al
J Diabetes Investig (2022)
(3) "CD73 in small extracellular vesicles derived from HNSCC defines tumour-associated immunosuppression mediated by macrophages in the microenvironment"
Lu, Zhang, Zhang et al
J Extracell Vesicles (2022) 11 (5), e12218
Showing 1-3 of 73421 papers.