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Recombinant Human SPAM1/PH20 hyaluronidase Protein,  His Tag (active enzyme) DMF Filed, 200µg  

Recombinant Human SPAM1/PH20 hyaluronidase Protein, His Tag (active enzyme) DMF Filed, 200µg

Recombinant Human SPAM1 /PH20 Protein, Leu 36 - Tyr 482, produced in human 293 cells (HEK293), His Tag (active enzyme) DMF Filed

Synonym
recombinant, human, protein, SPAM1, PH-20, HYAL3, HYA1, HYAL1, HYAL5, SPAG15

More details

PH0-H5225-200

Availability: within 7 days

1 250,00 €

Background
Hyaluronidase PH-20 also known as Sperm adhesion molecule 1 (SPAM1) and Sperm surface protein PH-20, whichbelongs to the glycosyl hydrolase 56 family, SPAM1 / PH-20 is expressed in testis. SPAM-1 / PH20 random hydrolysis of (1->4)-linkages between N – acetyl – beta – D – glucosamine and D-glucuronate residues in hyaluronate. SPAM-1 / PH20 involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. SPAM1 aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.

Source
Recombinant Human SPAM1 /PH20 Protein (rhPH20), His Tag (PH0-H5225) is expressed from human 293 cells (HEK293). It contains AA Leu 36 - Tyr 482 (Accession # P38567-1).
Predicted N-terminus: Leu 36

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 53.0 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhPH20 by the LAL method.

Purity
>95% purity as determined by SDS-PAGE of reduced rhPH20.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM NaCl, pH7.0-7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Site-specific integration as an efficient method for production of recombinant human hyaluronidase PH20 in semi-adherent cells"
Ata-Abadi, Forouzanfar, Dormiani et al
Appl Microbiol Biotechnol (2022)
(2) "Long-Term Outcomes in Adult Patients With Pulmonary Hypertension After Percutaneous Closure of Atrial Septal Defects"
Akseer, Abrahamyan, Lee et al
Circ Cardiovasc Interv (2022) 15 (1), e011110
(3) "Safety of recombinant human hyaluronidase PH20 for subcutaneous drug delivery"
Knowles, Printz, Kang et al
Expert Opin Drug Deliv (2021) 18 (11), 1673-1685
Showing 1-3 of 89 papers