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Anti-SARS-CoV-2 Neutralizing Antibody Titer Serologic Assay Kit, 96 tests  

Anti-SARS-CoV-2 Neutralizing Antibody Titer Serologic Assay Kit, 96 tests

Anti-SARS-CoV-2 Neutralizing Antibody Titer Serologic Assay Kit

This assay kit is used to measure the levels of Anti-SARS-CoV-2 neutralizing antibody which interfere ACE2/S protein RBD interaction. It is for research use only.

More details

TAS-K022-96tests

Availability: within 7 days

638,00 €

Background
The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) poses continual threat to human health due to rapid transmission worldwide. The Anti SARS-CoV-2 Neutralizing Antibody Titer Serologic Assay Kit to test the protective neutralizing antibody levels in post-vaccination serum or convalescent serum.

Application
This assay kit is used to measure the levels of Anti-SARS-CoV-2 neutralizing antibody which interfere ACE2/S protein RBD interaction. It is for research use only.

Reconstitution
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
The unopened kit is stable for at least 1 year from the date of manufacture if stored at 2°C to 8°C, and the opened kit is stable for up to 1 month from the date of opening at 2°C to 8°C.
Shipped at room temperature conditions.

Please note: Product is shipped on blue ice !

Assay Principles
The Anti-SARS-CoV-2 Neutralizing Antibody Titer Serologic Assay kit is a competitive ELISA. Immobilized Human ACE2 protein on the microplate are incubated with samples and Spike RBD-HRP. Presence of anti-SARS-CoV-2 neutralizing antibody in the samples competes with immobilized Human ACE2 protein to bind Spike RBD-HRP. The intensity of assay signal decreases proportionally to the presence of Anti-SARS-CoV-2 neutralizing antibody.

The kit analysis includes 5 simple steps:

a) All reagents are warmed to room temperature (20℃-25℃) before use.

b) Series of sample and control antibody dilution are prepared with dilution buffer. Solution of Spike RBD-HRP is prepared with dilution buffer.

c) The sample dilution is added to plate followed by Spike RBD-HRP solution. For semiquantitative analysis, dilution of control neutralizing antibody is add to the plate followed by Spike RBD-HRP solution.

d) After incubation, plates are washed and TMB or other colorimetric HRP substrate is addded.

e) The substrate reaction is stopped by add diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 650 nm to remove background noise prior to statistical analysis. The OD Value reflects the amount of protein bound.