Recombinant Human IL-2 Protein, Tag Free, 100 µg

Background:
Interleukin-2 (IL-2) is an interleukin, a type of cytokine immune system signaling molecule, which is a leukocytotrophic hormone that is instrumental in the body's natural response to microbial infection and in discriminating between foreign (non-self) and self. IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes, the cells that are responsible for immunity. Mature human IL-2 shares 56% and 66% aa sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit crossspecies activity. The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes. IL-2 is also necessary during T cell development in the thymus for the maturation of a unique subset of T cells that are termed regulatory T cells (T-regs). After exiting from the thymus, T-Regs function to prevent other T cells from recognizing and reacting against "self antigens", which could result in "autoimmunity". T-Regs do so by preventing the responding cells from producing IL-2. Thus, IL-2 is required to discriminate between self and non-self, another one of the unique characteristics of the immune system.

Source
Recombinant Human IL-2, Tag Free (IL2-H4113) is expressed from E.coli cells. It contains AA Ala 21 - Thr 153 (Accession # P60568-1).
Predicted N-terminus: Ala 21

Molecular Characterization
This protein carries no "tag".
The protein has a calculated MW of 15.4 kDa. The protein migrates as 15 kDa under reducing (R) condition (SDS-PAGE).

Endotoxin
Less than 0.1 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 20 mM PB, pH3.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Clinical Effect of Metronidazole Vaginal Effervescent Tablet Combined with Flavescentis Sophora Suppository in the Treatment of Trichomonas Vaginitis"
Shi, Yang, Wang
Contrast Media Mol Imaging (2022) 2022, 1250755
(2) "Dietary selenium sources alleviate immune challenge induced by Salmonella Enteritidis potentially through improving the host immune response and gut microbiota in laying hens"
Kang, Wang, Liu et al
Front Immunol (2022) 13, 928865
(3) "Road testing new CAR design strategies in multiple myeloma"
Rana, Murphy, Kort et al
Front Immunol (2022) 13, 957157
Showing 1-3 of 86485 papers.

Citations

(1) "Dual-optofluidic waveguide in-line fiber biosensor for real-time label-free detection of interferon-gamma with temperature compensation"
Authors: Gao R, et al
Journal: Opt Express 2020
Application: cross activity