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Recombinant Human PD-L1 / B7-H1 Protein (HPLC-verified), His tag, 1mg  

Recombinant Human PD-L1 / B7-H1 Protein (HPLC-verified), His tag, 1mg

Recombinant Human PD-L1 / B7-H1 Protein, Phe 19 - Arg 238, produced in human 293 cells (HEK293), (MALS-verified), His tag

Synonym
recombinant human protein PD-L1,CD274,B7-H1,PDCD1L1,PDCD1LG1

More details

PD1-H5229-1K

Availability: within 7 days

2 200,00 €

Background
Programmed cell death 1 ligand 1 (PDL1) is also known as B7-H, B7H1, MGC142294, MGC142296, PD-L1, PDCD1L1 and PDCD1LG1,which is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses.PDL1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. This protein is broadly expressed in the majority of peripheral tissues as well as hematopoietic cells. Interaction between PDL1 and its receptors belonging to the CD28 family of molecules provide both stimulatory and inhibitory signals in regulating T cell activation and tolerance. PDL1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression.

Source
Recombinant Human PD-L1, His Tag (PD1-H5229) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # NP_054862.1).
Predicted N-terminus: Phe 19

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 26.0 kDa. The protein migrates as 30-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the Human PD-L1, His Tag (HPLC-verified) by the LAL method.

Purity
>98% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization. 

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "CRISPR activation screen identifies BCL-2 proteins and B3GNT2 as drivers of cancer resistance to T cell-mediated cytotoxicity"
Joung, Kirchgatterer, Singh et al
Nat Commun (2022) 13 (1), 1606
(2) "DNA methylation profiles differ in responders versus non-responders to anti-PD-1 immune checkpoint inhibitors in patients with advanced and metastatic head and neck squamous cell carcinoma"
Starzer, Heller, Tomasich et al
J Immunother Cancer (2022) 10 (3)
(3) "Comparison of the immune activation capacities of fucoidan and laminarin extracted from Laminaria japonica"
An, Hwang, Kim et al
Int J Biol Macromol (2022)
Showing 1-3 of 19373 papers.

Citations

(1) "Generation of a safe and efficacious llama single-domain antibody fragment (vHH) targeting the membrane-proximal region of 4-1BB for engineering therapeutic bispecific antibodies for cancer"
Authors: Zhai T, Wang C, Xu Y, et al.
Journal: J Immunother Cancer 2021
Application: Cell based blocking assay
(2) "Heterocyclic compounds as immunomodulators"
Authors: L Wu, J Li, W Yao
Journal: US10669271B2 2020
Application: Binding Assay
(3) "Tumor-selective blockade of CD47 signaling with a CD47/PD-L1 bispecific antibody for enhanced anti-tumor activity and limited toxicity"
Authors: Wang Y, Ni H, Zhou S, et al.
Journal: Cancer Immunol Immunother 2020
Application: BLI
Showing 1-3 of 13 papers.


 

 

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